IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Colorimetric Cell-Based ELISA Kit

CHUK ELISA kit, phospho-specific, cell-based

The IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177).

Product Info Summary

SKU: EKC2043
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Colorimetric Cell-Based ELISA Kit

See all CHUK products

SKU/Catalog Number

EKC2043

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) protein expression profile in cells. The kit can be used for measuring the relative amounts of IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2043)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2043 is reactive to CHUK in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2043 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-IKK-alpha /IKK-beta Phospho-Ser177 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-IKK-alpha /IKK-beta Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CHUK (Source: Uniprot.Org, NCBI)

Gene Name

CHUK

Full Name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Weight

84640 MW

Superfamily

protein kinase superfamily

Alternative Names

CHUK;Conserved helix-loop-helix ubiquitous kinase;I kappa-B kinase alpha;I-kappa-B kinase 1;IkappaB kinase;IkBKA;IKK-A;IKK-alpha;IKK1;IKKA;Inhibitor of nuclear factor kappa-B kinase alpha subunit;kinase IKK-alpha;NFKBIKA;Nuclear factor NFkappaB inhibitor kinase alpha;I-kappa-B kinase 2;I-kappa-B-kinase beta;IkBKB;IKK-B;IKK-beta;IKK2;IKKB;kinase IKK-beta;NFKBIKB;Nuclear factor NF-kappa-B inhibitor kinase beta CHUK IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16 component of inhibitor of nuclear factor kappa B kinase complex inhibitor of nuclear factor kappa-B kinase subunit alpha|I-kappa-B kinase 1|I-kappa-B kinase-alpha|IKK-a kinase|IkB kinase alpha subunit|Nuclear factor NFkappaB inhibitor kinase alpha|TCF-16|conserved helix-loop-helix ubiquitous kinase|transcription factor 16

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHUK, check out the CHUK Infographic

CHUK infographic

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In this infographic you will see the following information for CHUK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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