Anti-DLAT Antibody Picoband®

Pyruvate Dehydrogenase E2 antibody

Boster Bio Anti-DLAT Antibody Picoband® catalog # A04469-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A04469-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-DLAT Antibody Picoband®

View all Pyruvate Dehydrogenase E2 Antibodies

SKU/Catalog Number

A04469-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DLAT Antibody Picoband® catalog # A04469-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DLAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04469-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DLAT recombinant protein (Position: P69-P642).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04469-2 is reactive to DLAT in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

69 kDa

Calculated molecular weight

128770 MW

Background of Pyruvate Dehydrogenase E2

Dihydrolipoyl transacetylase (or dihydrolipoamide acetyltransferase) is an enzyme component of the multienzyme pyruvate dehydrogenase complex. This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04469-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human Hela whole cell, human HEPG2 whole cell, human K562 whole cell, human T-47D whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue, mouse testis tissue, mouse NIH/3T3 whole cell
IHC: human liver cancer tissue, human placenta tissue, human renal clear cell carcinoma tissue, mouse brain tissue, rat brain tissue
ICC/IF: Caco-2 cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For DLAT (Source: Uniprot.org, NCBI)

Gene Name

DLAT

Full Name

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Weight

128770 MW

Superfamily

2-oxoacid dehydrogenase family

Alternative Names

Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain;CD11a;ITGAL;CD11A; DLAT DLTA, E2, PBC, PDC-E2, PDCE2 dihydrolipoamide S-acetyltransferase dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial|70 kDa mitochondrial auto of primary biliary cirrhosis|E2 component of pyruvate dehydrogenase complex|M2 complex 70 kDa subunit|dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex|pyruvate dehydrogenase complex component E2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DLAT, check out the DLAT Infographic

DLAT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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2 Customer Q&As for Anti-DLAT Antibody Picoband®

Question

What does DLAT stands for in A04469-2?

Verified customer

Asked: 2022-05-24

Answer

DLAT in the Anti-DLAT Antibody Picoband™ (A04469-2) stands for Dihydrolipoyl transacetylase.

Boster Scientific Support

Answered: 2022-05-24

Question

Is DLAT same as PDC-E2 antibody?

Verified customer

Asked: 2022-05-24

Answer

This gene in the in the Anti-DLAT Antibody Picoband™ (A04469-2) encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A.

Boster Scientific Support

Answered: 2022-05-24

Order DetailsPrice
A04469-2

100μg

$370
A04469-2-10ug

10μg sample (liquid)

$99
A04469-2-Biotin

100 μg Biotin conjugated

$570
A04469-2-Cy3

100 μg Cy3 conjugated

$570
A04469-2-Dylight488

100 μg Dylight488 conjugated

$570
A04469-2-Dylight550

100 μg Dylight550 conjugated

$570
A04469-2-Dylight594

100 μg Dylight594 conjugated

$570
A04469-2-FITC

100 μg FITC conjugated

$570
A04469-2-HRP

100 μg HRP conjugated

$570
A04469-2-APC

100 μg APC conjugated

$670
A04469-2-PE

100 μg PE conjugated

$670
A04469-2-iFluor647

100 μg iFluor647 conjugated

$670
A04469-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A04469-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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