Product Info Summary
SKU: | A01736-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-DIS3 Antibody
SKU/Catalog Number
A01736-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DIS3 Antibody catalog # A01736-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DIS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01736-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DIS3 recombinant protein (Position: Q62-A304).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01736-1 is reactive to DIS3 in Human, Rat
Applications
A01736-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
109 kDa
Calculated molecular weight
57954 MW
Background of DIS3
Exosome complex exonuclease RRP44 or Dis3 is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the nucleus of eukaryotic cells. By genomic sequence analysis, this gene is mapped to chromosome 13q21-q22. Expression of human DIS3 could partially complement Dis3 depletion in yeast.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
![a01736 1 dis3 primary antibodies wb testing 1 a01736 1 dis3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01736-1-dis3-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of DIS3 using anti-DIS3 antibody (A01736-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIS3 antigen affinity purified polyclonal antibody (Catalog # A01736-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DIS3 at approximately 109 kDa. The expected band size for DIS3 is at 109 kDa.
![a01736 1 dis3 primary antibodies if testing 2 a01736 1 dis3 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01736-1-dis3-primary-antibodies-if-testing-2.jpg)
Click image to see more details
Figure 2. IF analysis of DIS3 using anti-DIS3 antibody (A01736-1) and anti-Beta Tubulin antibody (M01857-3).
DIS3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DIS3 Antibody (A01736-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a01736 1 dis3 primary antibodies fcm testing 3 a01736 1 dis3 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01736-1-dis3-primary-antibodies-fcm-testing-3.jpg)
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Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-DIS3 antibody (A01736-1).
Overlay histogram showing MCF-7 cells stained with A01736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIS3 Antibody (A01736-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DIS3 (Source: Uniprot.org, NCBI)
Gene Name
DIS3
Full Name
Exosome complex exonuclease RRP44
Weight
57954 MW
Superfamily
RNR ribonuclease family
Alternative Names
2810028N01Rik; DIS3 mitotic control homolog (S. cerevisiae); dis3p; DKFZp667L1817; EC 3.1.13; EC 3.1.13.-; EC 3.1.26.-; EXOSC11; exosome complex exonuclease RRP44; exosome component 11; FLJ10484; KIAA1008RP11-342J4.3; MGC33035; mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; RRP44bA555G22.1 DIS3 2810028N01Rik, EXOSC11, KIAA1008, RRP44, dis3p DIS3 homolog, exosome endoribonuclease and 3-5 exoribonuclease exosome complex exonuclease RRP44|DIS3 exosome endoribonuclease and 3-5 exoribonuclease|DIS3 mitotic control homolog|exosome component 11|mitotic control protein dis3 homolog|ribosomal RNA-processing protein 44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DIS3, check out the DIS3 Infographic
![DIS3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DIS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DIS3 Antibody (A01736-1)
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Customer Q&As
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3 Customer Q&As for Anti-DIS3 Antibody
Question
Is a blocking peptide available for product anti-DIS3 antibody (A01736-1)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-DIS3 antibody (A01736-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-DIS3 antibody A01736-1 work on goat IHC-P with brain peripheral blood leukocyte?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Our lab technicians have not validated anti-DIS3 antibody A01736-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-DIS3 antibody A01736-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain peripheral blood leukocyte in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-DIS3 antibody A01736-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
The anti-DIS3 antibody (A01736-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-28