This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
3 Q&As
Facts about Exosome complex exonuclease RRP44.
The RNA exosome could be involved in Ig class switch recombination (CSR) or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is included in overall mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) in their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs.
Human | |
---|---|
Gene Name: | DIS3 |
Uniprot: | Q9Y2L1 |
Entrez: | 22894 |
Belongs to: |
---|
RNR ribonuclease family |
2810028N01Rik; DIS3 mitotic control homolog (S. cerevisiae); dis3p; DKFZp667L1817; EC 3.1.13; EC 3.1.13.-; EC 3.1.26.-; EXOSC11; exosome complex exonuclease RRP44; exosome component 11; FLJ10484; KIAA1008RP11-342J4.3; MGC33035; mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; RRP44bA555G22.1
Mass (kDA):
109.003 kDA
Human | |
---|---|
Location: | 13q21.33 |
Sequence: | 13; NC_000013.11 (72752169..72781900, complement) |
Widely expressed.
Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Predominantly located in the nucleus (PubMed:20531386). According to PubMed:12429849, found in the nucleolus (PubMed:12429849). According to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 (PubMed:20531386).
You have come to the right spot if you're looking high-affinity primary antibodies. Boster Bio is a leader in offering these antibodies, and their DIS3 Marker is no different. This article will discuss the DIS3 Marker and its uses. Continue reading to learn about the DIS3 Marker. You might even be surprised at the number of applications that this marker has in the field of immunohistochemistry.
Boster Bio has many products that can be used to test your flowcytometry analysis. These high-affinity antibodies are available in both monoclonal as well as polyclonal forms. Boster antibodies are well-known for their high citations. They have been used for more than 25years. Boster Bio is the best place to go if you're looking for antibodies that can test the expression of DIS3.
The DIS3 gene encodes a protein which regulates activity of an RNA exosome cluster. This complex is involved with mRNA turnover in general and in the degrading of inherently unstable mRNAs. DIS3 plays a role in the degrading of histone mRNA and in gene regulation. This prevents aberrant mRNA transcription. The gene is also responsible the stability of RNA polymerase II (subunit of ribosome complex), which is a component the RNA exosome complex.
The DIS3 marker may be used to determine if a specific gene is present. Its degeneration patterns will be similar if the gene expression is in its full-length form or in its PIN domain form. For proper functioning, the enzyme needs aspartic acids residues in its active center. Here we will discuss the most effective uses of the DIS3 indicator. Below are some examples of its use. You can also read more about the pharmacological activity.
DIS3 (a central component of exosome) is a crucial component. It is an integral part of the exosome and performs its endonucleolytic functions. The active site of Dis3 can be found close to the RING Channel of the exosome. The channel's top layer is stacked with RNA suggesting that the DIS3 markers has two roles in the RNA-degradation process. The mutation of Rrp41's basic residues can cause channel blockage. Mutation in the Rrp41 conserved basic amino acids results in decreased exosome activity, which correlates with a lack RNA protection.
RNAi is often used to study the exosome structure in human cells. Although RNAi is a fast and indirect way to reduce the effects of compensatory mechanisms, it provides an excellent tool to identify acute substrates. Rapid protein loss allows us to predict the frequency of such processes. Rapid protein depletion is more informative than slow, gradual protein loss over days. This method allows for the rapid detection of many neuronal substrates in a short time.
Among other uses for DIS3, this marker is used often to identify tumor cells. It is useful for studies that require cells transfected with RNAi. However it cannot be used to replace human cancer patients. DIS3–AID has many clinical uses because of its widespread use. This protein plays a role in the regulation and development of tumor growth.
The company's initial product, Steven Boster was released in 1993. The book store shelves were flooded with it and it quickly became a sensation. Boster, nicknamed "he who converts science in the lavatory," developed hundreds of primary antibodies and developed a proprietary ELISA platform. His company now has the largest Chinese antibody catalog. It also has proprietary trade secrets for high-sensitivity ELISA kits.
This book is divided up into three parts. Each part focuses on one aspect of slavery in South America. The author begins by recounting the story of Tom Wilson the disabled fugitive slave. He was used in abolitionist propaganda. The author then connects selling slaves to potential buyers to the display and exploitation of disabled fugitives. The reader can then understand the differences between disabled fugitives or slaves.
PMID: 9562621 by Shiomi T., et al. Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3.
PMID: 11935316 by Rozenblum E., et al. A genomic map of a 6-Mb region at 13q21-q22 implicated in cancer development: identification and characterization of candidate genes.