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Product Info Summary
| SKU: | A07378 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DIA-2 DIAPH2 Antibody
SKU/Catalog Number
A07378
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-DIA-2 DIAPH2 Antibody (Catalog # A07378). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-DIA-2 DIAPH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07378)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Standard Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07378 is reactive to DIAPH2 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
125569 MW
Background of DIAPH-2
DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described. DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07378 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:12,000 - 1:60,000
IP: 1:100
WB: 1:1,000 - 1:6,000
Validation Images & Assay Conditions
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Western blot analysis of DIA-2 expression in HEK293 cell lysates (lane 1). DIA-2 at 132KD was detected using rabbit anti-DIA-2 Antigen Affinity purified polyclonal antibody (Catalog # A07378) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For DIAPH2 (Source: Uniprot.org, NCBI)
Gene Name
DIAPH2
Full Name
Protein diaphanous homolog 2
Weight
125569 MW
Superfamily
formin homology family
Alternative Names
DIA2; DIAdiaphanous (Drosophila, homolog) 2; DIAPH2; DIAPH-2; diaphanous homolog 2 (Drosophila); Diaphanous-related formin-2; diaphorase-2; DRF2; POF; POF2; POF2FLJ11167; protein diaphanous homolog 2 DIAPH2 DIA, DIA2, DRF2, POF, POF2, POF2A diaphanous related formin 2 protein diaphanous homolog 2|diaphanous homolog 2|diaphorase-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DIAPH2, check out the DIAPH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DIAPH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DIA-2 DIAPH2 Antibody (A07378)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-DIA-2 DIAPH2 Antibody
Question
Would A07378 anti-DIA-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can see on the product datasheet, A07378 anti-DIA-2 antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-21
Question
My question regarding product A07378, anti-DIA-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07378 anti-DIA-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-DIA-2 antibody A07378. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
We are currently using anti-DIA-2 antibody A07378 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
C. Anderson
Verified customer
Asked: 2019-02-22
Answer
The anti-DIA-2 antibody (A07378) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-22
Question
Would anti-DIA-2 antibody A07378 work on feline WB with placenta?
J. Gonzalez
Verified customer
Asked: 2017-12-22
Answer
Our lab technicians have not tested anti-DIA-2 antibody A07378 on feline. You can run a BLAST between feline and the immunogen sequence of anti-DIA-2 antibody A07378 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-22
Question
Would anti-DIA-2 antibody A07378 work for IP with placenta?
M. Jones
Verified customer
Asked: 2014-03-04
Answer
According to the expression profile of placenta, DIAPH2 is highly expressed in placenta. So, it is likely that anti-DIA-2 antibody A07378 will work for IP with placenta.
Boster Scientific Support
Answered: 2014-03-04
Question
Here is the WB image, lot number and protocol we used for placenta using anti-DIA-2 antibody A07378. Please let me know if you require anything else.
J. Yang
Verified customer
Asked: 2013-04-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-22
