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Product Info Summary
| SKU: | A07520 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FMN1 Antibody Picoband®
SKU/Catalog Number
A07520
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FMN1 Antibody Picoband® catalog # A07520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FMN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07520)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419). Human FMN1 shares 93.8% amino acid (aa) sequence identity with mouse FMN1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07520 is reactive to FMN1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
158 kDa
Calculated molecular weight
157578 MW
Background of Fmn1
Formins, such as FMN1, are actin-nucleating proteins involved in cell polarity, cytokinesis, cell migration, and transcriptional activity. This FMN1 gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07520 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human,
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: mouse testis tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 2. IHC analysis of FMN1 using anti-FMN1 antibody (A07520).
FMN1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FMN1 Antibody (A07520) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of FMN1 using anti-FMN1 antibody (A07520).
FMN1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FMN1 Antibody (A07520) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of FMN1 using anti-FMN1 antibody (A07520).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMN1 antigen affinity purified polyclonal antibody (Catalog # A07520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMN1 at approximately 158KD. The expected band size for FMN1 is at 158KD.
Protein Target Info & Infographic
Gene/Protein Information For FMN1 (Source: Uniprot.org, NCBI)
Gene Name
FMN1
Full Name
Formin-1
Weight
157578 MW
Superfamily
formin homology family
Alternative Names
Formin-1;Limb deformity protein homolog;FMN1;FMN, LD; Fmn1|Fm, Fmn, form, formin-1, ld|formin 1|formin-1|limb deformity protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FMN1, check out the FMN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FMN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FMN1 Antibody Picoband® (A07520)
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Customer Q&As
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4 Customer Q&As for Anti-FMN1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-FMN1 antibody (A07520)?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-FMN1 antibody (A07520). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
We are currently using anti-FMN1 antibody A07520 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-FMN1 antibody (A07520) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
I have attached the WB image, lot number and protocol we used for retina using anti-FMN1 antibody A07520. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Does anti-FMN1 antibody A07520 work on bovine WB with brain?
O. Mitchell
Verified customer
Asked: 2013-11-19
Answer
Our lab technicians have not validated anti-FMN1 antibody A07520 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FMN1 antibody A07520 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-19
