Anti-FMN1 Antibody Picoband™

Product Name

Anti-FMN1 Antibody Picoband™

View all Fmn1 Antibodies

SKU/Catalog Number

A07520

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FMN1 Antibody Picoband™ catalog # A07520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FMN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07520)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419). Human FMN1 shares 93.8% amino acid (aa) sequence identity with mouse FMN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07520 is reactive to Fmn1 in Human, Mouse, Rat

Applications

A07520 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

158 kDa

Calculated molecular weight

157578 MW

Background of Fmn1

Formins, such as FMN1, are actin-nucleating proteins involved in cell polarity, cytokinesis, cell migration, and transcriptional activity. This FMN1 gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human,

Validation Images & Assay Conditions

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Figure 2. IHC analysis of FMN1 using anti-FMN1 antibody (A07520).
FMN1 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FMN1 Antibody (A07520) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of FMN1 using anti-FMN1 antibody (A07520).
FMN1 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FMN1 Antibody (A07520) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 1. Western blot analysis of FMN1 using anti-FMN1 antibody (A07520).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMN1 antigen affinity purified polyclonal antibody (Catalog # A07520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMN1 at approximately 158KD. The expected band size for FMN1 is at 158KD.

Gene/Protein Information For Fmn1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686C2281; DKFZp686G2387; FLJ45135; FMNLimb deformity protein homolog; formin (limb deformity); formin 1; formin-1; LDDKFZP686C2281; MGC125288; MGC125289 Fmn1|Fm, Fmn, form, formin-1, ld|formin 1|formin-1|limb deformity protein

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