Anti-DHODH Antibody Picoband® (monoclonal, 4E3)

DHODH antibody

Boster Bio Anti-DHODH Antibody Picoband® (monoclonal, 4E3) catalog # M04035. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M04035
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-DHODH Antibody Picoband® (monoclonal, 4E3)

View all DHODH Antibodies

SKU/Catalog Number

M04035

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DHODH Antibody Picoband® (monoclonal, 4E3) catalog # M04035. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DHODH Antibody Picoband® (monoclonal, 4E3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04035)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

4E3

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DHODH, different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04035 is reactive to DHODH in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

43 kDa

Calculated molecular weight

42.867kDa

Background of DHODH

Dihydroorotate dehydrogenase (DHODH) is an enzyme that in humans is encoded by the DHODH gene on chromosome 16. The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04035 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

Positive Control

WB: human Hela whole cell, human A431 whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat testis tissue, rat liver tissue, mouse testis tissue, mouse liver tissue
ICC/IF: MCF-7 cell
FCM: U937 cell

Validation Images & Assay Conditions

Gene/Protein Information For DHODH (Source: Uniprot.org, NCBI)

Gene Name

DHODH

Full Name

Dihydroorotate dehydrogenase (quinone), mitochondrial

Weight

42.867kDa

Superfamily

dihydroorotate dehydrogenase family

Alternative Names

SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2, UBLE1B; HRIHFB2115 DHODH DHOdehase, POADS, URA1 dihydroorotate dehydrogenase (quinone) dihydroorotate dehydrogenase (quinone), mitochondrial|dihydroorotate oxidase|human complement of yeast URA1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DHODH, check out the DHODH Infographic

DHODH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DHODH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M04035

100μg

$370
M04035-10ug

10μg sample (liquid)

$99
M04035-Biotin

100 μg Biotin conjugated

$570
M04035-Cy3

100 μg Cy3 conjugated

$570
M04035-Dylight488

100 μg Dylight488 conjugated

$570
M04035-Dylight550

100 μg Dylight550 conjugated

$570
M04035-Dylight594

100 μg Dylight594 conjugated

$570
M04035-FITC

100 μg FITC conjugated

$570
M04035-HRP

100 μg HRP conjugated

$570
M04035-APC

100 μg APC conjugated

$670
M04035-PE

100 μg PE conjugated

$670
M04035-iFluor647

100 μg iFluor647 conjugated

$670
M04035-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M04035
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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