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Product Info Summary
| SKU: | PB9801 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DGAT1 Antibody Picoband®
SKU/Catalog Number
PB9801
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DGAT1 Antibody Picoband® catalog # PB9801. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DGAT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9801)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DGAT1, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9801 is reactive to DGAT1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
55278 MW
Background of DGAT1
Acyl-CoA: diacylglycerol acyltransferase (DGAT) is a microsomal enzyme that plays a central role in the metabolism of cellular diacylglycerol lipids and catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT had been considered necessary for adipose tissue formation and essential for survival. There are two isozymes of DGAT encoded by the genes DGAT1 and DGAT2. DGAT1 is a host factor for HCV infection that binds core protein, localizes it to DGAT1-generated lipid droplets, and recruits viral RNA replication complexes for viral assembly. DGAT2-generated lipid droplets formed normally in cells treated with the DGAT1 inhibitor, suggesting that DGAT1 inhibitors may be useful as antiviral therapeutics.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9801 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Kidney Tissue, HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Kidney Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DGAT1 (Source: Uniprot.org, NCBI)
Gene Name
DGAT1
Full Name
Diacylglycerol O-acyltransferase 1
Weight
55278 MW
Superfamily
membrane-bound acyltransferase family
Alternative Names
Diacylglycerol O-acyltransferase 1;2.3.1.20 ;ACAT-related gene product 1;Acyl-CoA retinol O-fatty-acyltransferase;ARAT;Retinol O-fatty-acyltransferase;2.3.1.76 ;Diglyceride acyltransferase;DGAT1;AGRP1, DGAT; DGAT1 ARAT, ARGP1, DGAT, DIAR7 diacylglycerol O-acyltransferase 1 diacylglycerol O-acyltransferase 1|ACAT related gene product 1|acyl coenzyme A:cholesterol acyltransferase related gene 1|acyl-CoA retinol O-fatty-acyltransferase|acyl-CoA:diacylglycerol acyltransferase|diglyceride acyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGAT1, check out the DGAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DGAT1 Antibody Picoband® (PB9801)
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Customer Q&As
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7 Customer Q&As for Anti-DGAT1 Antibody Picoband®
Question
Do you have a BSA free version of anti-DGAT1 antibody PB9801 available?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DGAT1 antibody PB9801 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-12
Question
I am interested in to test anti-DGAT1 antibody PB9801 on human small intestine peyer's patch for research purposes, then I may be interested in using anti-DGAT1 antibody PB9801 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The products we sell, including anti-DGAT1 antibody PB9801, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine peyer's patch using anti-DGAT1 antibody PB9801. Let me know if you need anything else.
Z. Banerjee
Verified customer
Asked: 2019-05-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-DGAT1 antibody PB9801 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
The anti-DGAT1 antibody (PB9801) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-30
Question
Does PB9801 anti-DGAT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
It shows on the product datasheet, PB9801 anti-DGAT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-09
Question
I was wanting to use your anti-DGAT1 antibody for WB for human small intestine peyer's patch on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human small intestine peyer's patch identification?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
As indicated on the product datasheet, PB9801 anti-DGAT1 antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine peyer's patch in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-14
Question
I see that the anti-DGAT1 antibody PB9801 works with WB, what is the protocol used to produce the result images on the product page?
R. Brown
Verified customer
Asked: 2016-06-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-06-02
