This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Diacylglycerol O-acyltransferase 1.
May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol.
Human | |
---|---|
Gene Name: | DGAT1 |
Uniprot: | O75907 |
Entrez: | 8694 |
Belongs to: |
---|
membrane-bound acyltransferase family |
ACAT-related gene product 1; AGRP1; ARAT; ARGP1; ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1; DGAT; DGAT1; DGATACAT related gene product 1; diacylglycerol O-acyltransferase 1; diacylglycerol O-acyltransferase homolog 1 (mouse); diacylglycerol O-acyltransferase homolog 1; DIAR7; Diglyceride acyltransferase; EC 2.3.1; EC 2.3.1.20
Mass (kDA):
55.278 kDA
Human | |
---|---|
Location: | 8q24.3 |
Sequence: | 8; NC_000008.11 (144314584..144326852, complement) |
Endoplasmic reticulum membrane; Multi-pass membrane protein.
There are several steps in optimizing an ELISA experiment, including preparation of the sample, antibodies, blocking buffers, and more. Boster Bio optimization guides will answer your questions and assist you maximize the results of your experiment. Continue reading to find out more. This article will cover three of the most important topics when it comes to optimizing an ELISA experiment: subpopulations optimization and applications. Once you've mastered these steps, you'll be well on your way to conducting an effective ELISA experiment.
The DGAT1 gene encodes the enzyme acyl-CoA:diacylglycerol acyltransferase. Its polymorphism can be associated with milk yield or composition. This marker could aid researchers identify the gene responsible for TG biosynthesis. The gene is also associated with various other traits, such as cholesterol levels and dietary fiber.
DGAT1 is an enzyme in the family of sterol-o-acyltransferases. They catalyzes the last step of TG synthesizing. The DGAT1 enzyme fold is able to recognize hydrophilic CoA patterns and has separate entrances for the two substrates. It is an integral part of the membrane-bound Acyltransferase family. Boster Bio provides customized DNA sequencing services for research and development projects using Cas9 proteins.
Steven Boster, in the late nineteen nineties, came up with his first product, a variety IHC products. He created hundreds of primary antibodies, and his company became the largest Chinese catalog of antibodies. Boster bio also created proprietary ELISA platforms, including the PicoKine(tm) system. This platform integrates trade secrets to offer high-sensitivity ELISA kits that can detect DGAT1 or DGAT2.
The DGAT1 gene has been discovered on chromosomes 14, 15, and 9 of cattle. This gene plays a crucial role in fat metabolism specifically TAG synthesis. Its polymorphisms were studied in the past, and they can be used to breed dairy and beef cattle. However, the uses of this gene are still limited. Let's now look at some of its applications.
Real-time PCR is used to determine whether DGAT1 is present or not. Researchers can compare the abundances of full-length mRNA and alternatively sequenced mRNA. In the latter instance the alternatively spliced transcript was 100-fold less abundant than the full-length transcript. These findings are significant in many areas of biology including veterinary medicine.
It has been demonstrated that the DGAT1 gene also has an antigen binding property that makes it a suitable biomarker for research into cancer. The DGAT1 gene encodes a specific protein. The nucleic acid molecule that encodes DGAT1 contains an antigen-binding antigen and a reagent to measure the amount of protein in the sample. The antibody binds with the protein and enhances its detection.
Using the DGAT1 gene in cattle has many applications. It is currently being used in marker assisted selection to improve milk production traits in cattle. Diagnostic kits for bovine genetic material DGAT1 is available. The kit contains primers to aid in amplifying genetic material. However these primers are compatible to the normal DGAT1 gene. After the primers have been amplified and analyzed, the DGAT1 genes can be analyzed.
It has been shown that the DGAT1 gene may also impact milk yield and fat. Studies have shown that the these gene polymorphisms could be useful for the identification of genetic markers to improve the yield of milk and fat in cattle. The DGAT1 gene may also be useful in breeding cattle. If this gene is discovered in dairy cattle, the gene could enhance the quality of milk through improving the fat content in the meat and milk.
PMID: 9756920 by Oelkers P., et al. Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes.
PMID: 16214399 by Orland M.D., et al. Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1.