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Product Info Summary
| SKU: | M02035 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Desmoglein 2 Monoclonal Antibody
View all Desmoglein-2 Antibodies
SKU/Catalog Number
M02035
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Desmoglein 2 Monoclonal Antibody catalog # M02035. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Desmoglein 2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02035)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFBF-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Desmoglein 2 Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02035 is reactive to DSG2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
13157 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02035 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Desmoglein 2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DSG2 (Source: Uniprot.org, NCBI)
Gene Name
DSG2
Full Name
Desmoglein-2
Weight
13157 MW
Alternative Names
Melanoma antigen recognized by T-cells 1;MART-1;Antigen LB39-AA;Antigen SK29-AA;Protein Melan-A;MLANA;MART1; DSG2 CDHF5, HDGC desmoglein 2 desmoglein-2|cadherin family member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DSG2, check out the DSG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DSG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Desmoglein 2 Monoclonal Antibody (M02035)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Desmoglein 2 Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Desmoglein 2 Monoclonal antibody M02035. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-Desmoglein 2 Monoclonal antibody M02035 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to the expression profile of liver, DSG2 is highly expressed in liver. So, it is likely that anti-Desmoglein 2 Monoclonal antibody M02035 will work for WB with liver.
Boster Scientific Support
Answered: 2019-11-08
Question
I was wanting to use your anti-Desmoglein 2 Monoclonal antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
As indicated on the product datasheet, M02035 anti-Desmoglein 2 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Does anti-Desmoglein 2 Monoclonal antibody M02035 work on dog WB with cervix carcinoma erythroleukemia?
S. Taylor
Verified customer
Asked: 2018-12-06
Answer
Our lab technicians have not tested anti-Desmoglein 2 Monoclonal antibody M02035 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Desmoglein 2 Monoclonal antibody M02035 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-06
Question
I was wanting to use to test anti-Desmoglein 2 Monoclonal antibody M02035 on mouse liver for research purposes, then I may be interested in using anti-Desmoglein 2 Monoclonal antibody M02035 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
The products we sell, including anti-Desmoglein 2 Monoclonal antibody M02035, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-24
Question
I see that the anti-Desmoglein 2 Monoclonal antibody M02035 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-06
Question
Is this M02035 anti-Desmoglein 2 Monoclonal antibody reactive to the isotypes of DSG2?
R. Brown
Verified customer
Asked: 2014-01-16
Answer
The immunogen of M02035 anti-Desmoglein 2 Monoclonal antibody is A synthesized peptide derived from human Desmoglein 2 Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-16
