Anti-DEPTOR Antibody

Product Name

Anti-DEPTOR Antibody

View all DEPTOR/DEPDC6 Antibodies

SKU/Catalog Number

A03811

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DEPTOR Antibody (Catalog # A03811). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-DEPTOR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03811)

Host

Rabbit

Contents

DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.

Reactive Species

A03811 is reactive to DEPTOR in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

46294 MW

Background of DEPTOR/DEPDC6

DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03811 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DEPTOR (Source: Uniprot.org, NCBI)

Alternative Names

DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6 DEPTOR DEP.6, DEPDC6 DEP domain containing MTOR interacting protein DEP domain-containing mTOR-interacting protein|DEP domain containing 6|DEP domain-containing protein 6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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