Anti-DDX60 Antibody

Product Name

Anti-DDX60 Antibody

View all DDX60 Antibodies

SKU/Catalog Number

A09938

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DDX60 Antibody (Catalog # A09938). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DDX60 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DDX60 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09938)

Host

Rabbit

Contents

DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60. The immunogen is located within amino acids 1140 - 1190 of DDX60.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.

Reactive Species

A09938 is reactive to DDX60 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

197853 MW

Background of DDX60

DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09938 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DDX60 (Source: Uniprot.org, NCBI)

Alternative Names

Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60 DDX60 DExD/H-box helicase 60 probable ATP-dependent RNA helicase DDX60|ATP-dependent RNA helicase DDX60|DEAD (Asp-Glu-Ala-Asp) box polypeptide 60|DEAD box protein 60|DEAD-box helicase 60

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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