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Product Info Summary
| SKU: | M02448-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3)
SKU/Catalog Number
M02448-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3) catalog # M02448-1. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02448-1)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
3C3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02448-1 is reactive to DDX4 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
79 kDa
Calculated molecular weight
76.47kDa
Background of VASA
DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02448-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunofluorescence, 5 μg/ml, Mouse, Rat
Positive Control
WB: rat testis tissue, mouse testis tissue
IHC: mouse testis tissue, rat testis tissue
IF: mouse testis tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DDX4/MVH using anti-DDX4/MVH antibody (M02448-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DDX4/MVH antigen affinity purified monoclonal antibody (Catalog # M02448-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DDX4/MVH at approximately 79 kDa. The expected band size for DDX4/MVH is at 79 kDa.
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Figure 2. IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (M02448-1).
DDX4/MVH was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DDX4/MVH Antibody (M02448-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (M02448-1).
DDX4/MVH was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DDX4/MVH Antibody (M02448-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of DDX4/MVH using anti-DDX4/MVH antibody (M02448-1).
DDX4/MVH was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-DDX4/MVH Antibody (M02448-1) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of DDX4/MVH using anti-DDX4/MVH antibody (M02448-1).
DDX4/MVH was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-DDX4/MVH Antibody (M02448-1) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For DDX4 (Source: Uniprot.org, NCBI)
Gene Name
DDX4
Full Name
ATP-dependent RNA helicase DDX4
Weight
76.47kDa
Superfamily
DEAD box helicase family
Alternative Names
Peptidyl-prolyl cis-trans isomerase D; PPIase D Ddx4|AV206478, Mv, Mvh, VASA|DEAD box helicase 4|ATP-dependent RNA helicase DDX4|D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD (Asp-Glu-Ala-Asp) box polypeptide 4|DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD box polypeptide 4|DEAD box protein 4|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|mvh / mvasa|probable ATP-dependent RNA helicase DDX4|vasa homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX4, check out the DDX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX4/MVH Antibody Picoband® (monoclonal, 3C3) (M02448-1)
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