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Product Info Summary
| SKU: | PB9757 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-DDB2 Antibody Picoband®
SKU/Catalog Number
PB9757
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DDB2 Antibody Picoband® catalog # PB9757. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9757)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DDB2 recombinant protein (Position: M1-T115). Human DDB2 shares 58.3% amino acid (aa) sequence identity with mouse DDB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9757 is reactive to DDB2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
47864 MW
Background of XPE/DDB2
DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9757 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: A431 Whole Cell, SW620 Whole Cell, HELA Whole Cell, JURKAT Whole Cell
ICC/IF: U20S cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DDB2 using anti-DDB2 antibody (PB9757).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: A431 Whole Cell Lysate,
Lane 2: SW620 Whole Cell Lysate,
Lane 3: HELA Whole Cell Lysate,
Lane 4: JURKAT Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # PB9757) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.
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Figure 2. IF analysis of DDB2 using anti-DDB2 antibody (PB9757).
DDB2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDB2 Antibody (PB9757) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of 293T cells using anti-DDB2 antibody (PB9757).
Overlay histogram showing 293T cells stained with PB9757 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDB2 Antibody (PB9757,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DDB2 (Source: Uniprot.org, NCBI)
Gene Name
DDB2
Full Name
DNA damage-binding protein 2
Weight
47864 MW
Superfamily
WD repeat DDB2/WDR76 family
Alternative Names
DNA damage-binding protein 2;DDB p48 subunit;DDBb;Damage-specific DNA-binding protein 2;UV-damaged DNA-binding protein 2;UV-DDB 2;DDB2; DDB2 DDBB, UV-DDB2, XPE damage specific DNA binding protein 2 DNA damage-binding protein 2|DDB p48 subunit|UV-DDB 2|UV-damaged DNA-binding protein 2|damage-specific DNA binding protein 2, 48kDa|xeroderma pigmentosum group E protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDB2, check out the DDB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDB2 Antibody Picoband® (PB9757)
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Customer Q&As
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6 Customer Q&As for Anti-DDB2 Antibody Picoband®
Question
Would PB9757 anti-DDB2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
As indicated on the product datasheet, PB9757 anti-DDB2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-21
Question
Is a blocking peptide available for product anti-DDB2 antibody (PB9757)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We do provide the blocking peptide for product anti-DDB2 antibody (PB9757). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-07
Question
We are currently using anti-DDB2 antibody PB9757 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-DDB2 antibody (PB9757) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Would anti-DDB2 antibody PB9757 work on monkey WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
Our lab technicians have not validated anti-DDB2 antibody PB9757 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-DDB2 antibody PB9757 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-17
Question
Is this PB9757 anti-DDB2 antibody reactive to the isotypes of DDB2?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
The immunogen of PB9757 anti-DDB2 antibody is E.coli-derived human DDB2 recombinant protein (Position: M1-T115). Human DDB2 shares 58.3% amino acid (aa) sequence identity with mouse DDB2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-05
Question
My question regarding product PB9757, anti-DDB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Edwards
Verified customer
Asked: 2016-04-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9757 anti-DDB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-01
