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Product Info Summary
| SKU: | M08577 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-DCAF7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M08577
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-DCAF7 Rabbit Monoclonal Antibody catalog # M08577. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DCAF7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08577)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27D63
Isotype
IgG
Immunogen
A synthesized peptide derived from human DCAF7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08577 is reactive to DCAF7 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
39 kDa
Calculated molecular weight
50152 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08577 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat C6 whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DCAF7 using anti-DCAF7 antibody (M08577).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF7 antigen affinity purified monoclonal antibody (Catalog # M08577) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCAF7 at approximately 39 kDa. The expected band size for DCAF7 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DCAF7 (Source: Uniprot.org, NCBI)
Gene Name
DCAF7
Full Name
DDB1- and CUL4-associated factor 7
Weight
50152 MW
Superfamily
WD repeat DCAF7 family
Alternative Names
Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain;TUBA1B; DCAF7 AN11, HAN11, SWAN-1, WDR68 DDB1 and CUL4 associated factor 7 DDB1- and CUL4-associated factor 7|WD repeat-containing protein 68|WD repeat-containing protein An11 homolog|human anthocyanin|seven-WD-repeat protein of the AN11 family-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DCAF7, check out the DCAF7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DCAF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DCAF7 Rabbit Monoclonal Antibody (M08577)
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