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Product Info Summary
| SKU: | A07947-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-DAZAP1 Antibody Picoband®
SKU/Catalog Number
A07947-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DAZAP1 Antibody Picoband® catalog # A07947-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAZAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07947-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DAZAP1 recombinant protein (Position: M1-E189).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07947-1 is reactive to DAZAP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
49229 MW
Background of DAZAP1
DAZ-associated protein 1 is a protein that in humans is encoded by the DAZAP1 gene. It is mapped to19p13.3. In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07947-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human AGS whole cell, human GES-1 whole cell, human HGC-27 whole cell, human Hela whole cell, human Jurkat whole cell, human 293T whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell
IHC: mouse testis tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human AGS whole cell lysates,
Lane 2: human GES-1 whole cell lysates,
Lane 3: human HGC-27 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human Jurkat whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZAP1 antigen affinity purified polyclonal antibody (Catalog # A07947-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAZAP1 at approximately 43 kDa. The expected band size for DAZAP1 is at 43 kDa.
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Figure 2. IHC analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1).
DAZAP1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZAP1 Antibody (A07947-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of DAZAP1 using anti-DAZAP1 antibody (A07947-1).
DAZAP1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DAZAP1 Antibody (A07947-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DAZAP1 (Source: Uniprot.org, NCBI)
Gene Name
DAZAP1
Full Name
DAZ-associated protein 1
Weight
49229 MW
Alternative Names
DAZ-associated protein 1; Deleted in azoospermia-associated protein 1; DAZAP1 DAZAP1 DAZ associated protein 1 DAZ-associated protein 1|deleted in azoospermia-associated protein 1|testicular tissue protein Li 50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAZAP1, check out the DAZAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAZAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAZAP1 Antibody Picoband® (A07947-1)
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Customer Q&As
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4 Customer Q&As for Anti-DAZAP1 Antibody Picoband®
Question
I see that the anti-DAZAP1 antibody A07947-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-29
Question
We are currently using anti-DAZAP1 antibody A07947-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
M. Miller
Verified customer
Asked: 2019-01-28
Answer
The anti-DAZAP1 antibody (A07947-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-28
Question
I am looking for to test anti-DAZAP1 antibody A07947-1 on rat colon carcinoma for research purposes, then I may be interested in using anti-DAZAP1 antibody A07947-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Evans
Verified customer
Asked: 2019-01-23
Answer
The products we sell, including anti-DAZAP1 antibody A07947-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-23
Question
I have a question about product A07947-1, anti-DAZAP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Jones
Verified customer
Asked: 2013-01-21
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07947-1 anti-DAZAP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-21
