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- Table of Contents
Facts about DAZ-associated protein 1.
Human | |
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Gene Name: | DAZAP1 |
Uniprot: | Q96EP5 |
Entrez: | 26528 |
Belongs to: |
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No superfamily |
DAZ associated protein 1; DAZ-associated protein 1; deleted in azoospermia associated protein 1; Deleted in azoospermia-associated protein 1; MGC19907
Mass (kDA):
43.383 kDA
Human | |
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Location: | 19p13.3 |
Sequence: | 19; NC_000019.10 (1407586..1435684) |
Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm (By similarity).
This article will provide more information about the primary antibodies of Steven Boster. This article will give you information about his work and how His antibodies were used in a variety of applications. Flow Cytometry is an effective method for studying cells, particles and other biological systems. Boster produces monoclonal aswell as polyclonal primary antibodies that are highly affinity-based and have been widely cited for more than a quarter of a century.
DAZAP1 is a protein in the nuclear system that is extremely expressed in a variety of tissues. When you use antibodies against the protein, it is possible to detect changes in the cell cycle stages including anaphase and apoptosis. To use this antibody, HEK 293T cells were cultivated in conventional DMEM, RPMI, or puromycin media. It was tested by western blot to see if the antibody recognized DAZAP1.
DAZAP1 is a downstream substrate of the MEK/Erk signaling pathway. Over-expression of DAZAP1 inhibits cell proliferation, but knockdown reduces cell migration. Knockdown of DAZAP1 slows cell growth and proliferation without significantly altering the process of cell division. Therefore, rescue experiments using anti-DAZAP1 antibodies are challenging. Normal cell proliferation is contingent on the levels of the protein. DAZAP1 knockout mice showed the development delays and slowed growth rates.
DAZAP1 interacts with several RNA motifs, and forms a proteinprotein combination with hnRNPs. The protein binds to four distinct RNAs, the ISE in green and ISS in red and red, respectively. This interaction is determined using an SPR test using DAZAP1 purified protein as well as synthetic RNA oligos containing consensus motif.
DAZAP1 interacts with Shuttling splicing factors, such as SC35, and is located in the perinuclear/cytoplasmic region. DAZAP1 is distinct from nuclear speckles containing SC35 or SRSF2. DAZAP1 is targeted by Erk-mediated protein transferlation, unlike SRSF2.
Boster Bio, founded in 1993 by Steven Xia, is a pioneer in the development of high-specificity and sensitivity antibody. Their proprietary technology and techniques have helped them develop highly effective antibodies that are frequently referenced in more than 29,000 publications. All Boster Bio antibodies are approved for use in IHC and WB, ELISA and Flow Cytometry.
Primary antibodies are immunoglobulins that connect to a specific antigen. Primary antibodies are classified based on their specificity or affinity. This is a reference to the strength and the non-covalent bonds they create with their antigens of choice. High specificity antibodies have a high affinity to the antigen targeted and have lower levels of binding to other antigens. Primary antibodies are utilized for various purposes and can be measured, purified and detected.
Primary antibodies are the core of immunoassays and can be found in clinical and basic research environments. They are beneficial because they can recognize and bind to specific antigens. Examples of immunoassays are Western Blotting, immunohistochemistry, histopathology tests, and ELISA, or enzyme-mediated immunoassays. Flow cytometry, on the other hand, determines the abundance of certain cell kinds.
PMID: 10857750 by Tsui S., et al. Identification of two novel proteins that interact with germ-cell- specific RNA-binding proteins DAZ and DAZL1.
PMID: 22001406 by Sasaki K., et al. Acetylation of Prrp K150 regulates the subcellular localization.