Anti-Zebrafish Mmp2 Antibody

Product Name

Anti-Zebrafish Mmp2 Antibody

View all MMP-2 Antibodies

SKU/Catalog Number

DZ41117

Size

200 μl/vial

Form

Liquid

Description

Boster Bio Anti-Mmp2 Antibody catalog # DZ41117. This antibody reacts with Zebrafish.

Storage & Handling

At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.

Cite This Product

Anti-Zebrafish Mmp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ41117)

Host

Rabbit

Contents

Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RBM45 recombinant protein (Position: A2-R215+Q370-Y476).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

DZ41117 is reactive to MMP2 in Zebrafish

Applications

DZ41117 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

46663 MW

Background of MMP-2

Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5 μg/ml, Hu
Immunohistochemistry(Paraffin-embedded Section), 1 μg/ml, Hu

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of Mmp2 using anti-Mmp2 antibody (DZ41117).
Mmp2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mmp2 Antibody (DZ41117) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Click image to see more details

Figure 2. IHC analysis of Mmp2 using anti-Mmp2 antibody (DZ41117).
Mmp2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mmp2 Antibody (DZ41117) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For MMP2 (Source: Uniprot.org, NCBI)

Alternative Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) MMP2 CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 matrix metallopeptidase 2 72 kDa type IV collagenase|collagenase type IV-A|matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|matrix metalloproteinase-2|matrix metalloproteinase-II|neutrophil gelatinase

For more info on MMP2, check out the MMP2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MMP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MMP2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis