Product Info Summary
SKU: | A12154 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DAK TKFC Antibody
SKU/Catalog Number
A12154
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-DAK TKFC Antibody (Catalog # A12154). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DAK TKFC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12154)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human DAK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12154 is reactive to TKFC in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
58947 MW
Background of TKFC
Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
Ota T., Nat. Genet. 36:40-45(2004).
Totoki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12154 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells and A549 cells, using DAK antibody A12154.
Protein Target Info & Infographic
Gene/Protein Information For TKFC (Source: Uniprot.org, NCBI)
Gene Name
TKFC
Full Name
Triokinase/FMN cyclase
Weight
58947 MW
Superfamily
dihydroxyacetone kinase (DAK) family
Alternative Names
Triokinase/FMN cyclase ;Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing);ATP-dependent dihydroxyacetone kinase;DHA kinase;2.7.1.28;2.7.1.29;Glycerone kinase;Triokinase;Triose kinase;FAD-AMP lyase (cyclizing);4.6.1.15;FAD-AMP lyase (cyclic FMN forming);FMN cyclase;TKFC ;DAK ; TKFC DAK, NET45D, TKFC triokinase and FMN cyclase triokinase/FMN cyclase|ATP-dependent dihydroxyacetone kinase|DHA kinase|Dha kinase/FMN cyclase|FAD-AMP lyase cyclic FMN forming|FAD-AMP lyase cyclizing|bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)|dihydroxyacetone kinase 2 homolog|glycerone kinase|testis tissue sperm-binding protein Li 84P
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TKFC, check out the TKFC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TKFC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DAK TKFC Antibody (A12154)
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No publications found for A12154
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Customer Q&As
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5 Customer Q&As for Anti-DAK TKFC Antibody
Question
Is there a BSA free version of anti-DAK antibody A12154 available?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DAK antibody A12154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-07
Question
I see that the anti-DAK antibody A12154 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-06
Question
Will A12154 anti-DAK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can see on the product datasheet, A12154 anti-DAK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-15
Question
Is a blocking peptide available for product anti-DAK antibody (A12154)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-DAK antibody (A12154). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
I have a question about product A12154, anti-DAK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12154 anti-DAK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-26