Anti-DAK TKFC Antibody

TKFC antibody

Boster Bio Anti-DAK TKFC Antibody (Catalog # A12154). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A12154
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-DAK TKFC Antibody

View all TKFC Antibodies

SKU/Catalog Number

A12154

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-DAK TKFC Antibody (Catalog # A12154). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DAK TKFC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12154)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human DAK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12154 is reactive to TKFC in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

58947 MW

Background of TKFC

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.

Ota T., Nat. Genet. 36:40-45(2004).
Totoki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12154 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For TKFC (Source: Uniprot.org, NCBI)

Gene Name

TKFC

Full Name

Triokinase/FMN cyclase

Weight

58947 MW

Superfamily

dihydroxyacetone kinase (DAK) family

Alternative Names

Triokinase/FMN cyclase ;Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing);ATP-dependent dihydroxyacetone kinase;DHA kinase;2.7.1.28;2.7.1.29;Glycerone kinase;Triokinase;Triose kinase;FAD-AMP lyase (cyclizing);4.6.1.15;FAD-AMP lyase (cyclic FMN forming);FMN cyclase;TKFC ;DAK ; TKFC DAK, NET45D, TKFC triokinase and FMN cyclase triokinase/FMN cyclase|ATP-dependent dihydroxyacetone kinase|DHA kinase|Dha kinase/FMN cyclase|FAD-AMP lyase cyclic FMN forming|FAD-AMP lyase cyclizing|bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)|dihydroxyacetone kinase 2 homolog|glycerone kinase|testis tissue sperm-binding protein Li 84P

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TKFC, check out the TKFC Infographic

TKFC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TKFC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-DAK TKFC Antibody

Question

Is there a BSA free version of anti-DAK antibody A12154 available?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DAK antibody A12154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-05-07

Question

I see that the anti-DAK antibody A12154 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-06

Question

Will A12154 anti-DAK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

You can see on the product datasheet, A12154 anti-DAK antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-15

Question

Is a blocking peptide available for product anti-DAK antibody (A12154)?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We do provide the blocking peptide for product anti-DAK antibody (A12154). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-28

Question

I have a question about product A12154, anti-DAK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12154 anti-DAK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-26

Order DetailsPrice
A12154-100uL

100uL

$360

Free Secondary Antibody

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A12154
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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