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Product Info Summary
| SKU: | A14002 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-NEK5 Antibody
SKU/Catalog Number
A14002
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NEK5 Antibody (Catalog # A14002). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEK5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14002)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NEK5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14002 is reactive to NEK5 in Human
Reconstitution
Calculated molecular weight
81445 MW
Background of Nek5
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Dunham A., Nature 428:522-528(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14002 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and COLO205 cells, using NEK5 antibody A14002.
Protein Target Info & Infographic
Gene/Protein Information For NEK5 (Source: Uniprot.org, NCBI)
Gene Name
NEK5
Full Name
Serine/threonine-protein kinase Nek5
Weight
81445 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase Nek5;2.7.11.1;Never in mitosis A-related kinase 5;NimA-related protein kinase 5;NEK5;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEK5, check out the NEK5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEK5 Antibody (A14002)
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No publications found for A14002
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-NEK5 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for bronchial epithelial cell using anti-NEK5 antibody A14002. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
I was wanting to use your anti-NEK5 antibody for WB for human bronchial epithelial cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bronchial epithelial cell identification?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
As indicated on the product datasheet, A14002 anti-NEK5 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bronchial epithelial cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-23
Question
Is there a BSA free version of anti-NEK5 antibody A14002 available?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NEK5 antibody A14002 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-11-23
Question
Does A14002 anti-NEK5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Anderson
Verified customer
Asked: 2018-10-11
Answer
As indicated on the product datasheet, A14002 anti-NEK5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-11
Question
My question regarding product A14002, anti-NEK5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Huang
Verified customer
Asked: 2018-08-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14002 anti-NEK5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-27
Question
I am interested in to test anti-NEK5 antibody A14002 on human bronchial epithelial cell for research purposes, then I may be interested in using anti-NEK5 antibody A14002 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Edwards
Verified customer
Asked: 2015-01-27
Answer
The products we sell, including anti-NEK5 antibody A14002, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-01-27
