Product Info Summary
SKU: | A09234 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospholipase A1 member A PLA1A Antibody
SKU/Catalog Number
A09234
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospholipase A1 member A PLA1A Antibody catalog # A09234. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospholipase A1 member A PLA1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09234)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human PLA1A. at AA rangle: 350-430
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09234 is reactive to PLA1A in Human
Applications
A09234 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
49715 MW
Background of PLA1A
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
![a09234 western blotting analysis 1 a09234 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a09234-western-blotting-analysis-1.jpg)
Click image to see more details
Western Blot (WB) analysis of 3T3 cells using PLA1A Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PLA1A (Source: Uniprot.org, NCBI)
Gene Name
PLA1A
Full Name
Phospholipase A1 member A
Weight
49715 MW
Superfamily
AB hydrolase superfamily
Alternative Names
EC 3.1.1; EC 3.1.1.-; NMD; phosphatidylserine-specific phospholipase A1alpha; phospholipase A1 member A; PS-PLA1; PSPLA1Phosphatidylserine-specific phospholipase A1 PLA1A PS-PLA1, PSPLA1 phospholipase A1 member A phospholipase A1 member A|phosphatidylserine-specific phospholipase A1alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLA1A, check out the PLA1A Infographic
![PLA1A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospholipase A1 member A PLA1A Antibody (A09234)
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No publications found for A09234
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Customer Q&As
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5 Customer Q&As for Anti-Phospholipase A1 member A PLA1A Antibody
Question
I was wanting to use your anti-PLA1A antibody for WB for human corpus epididymis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human corpus epididymis identification?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
You can see on the product datasheet, A09234 anti-PLA1A antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human corpus epididymis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-16
Question
Does anti-PLA1A antibody A09234 work for WB with corpus epididymis?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
According to the expression profile of corpus epididymis, PLA1A is highly expressed in corpus epididymis. So, it is likely that anti-PLA1A antibody A09234 will work for WB with corpus epididymis.
Boster Scientific Support
Answered: 2020-03-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for corpus epididymis using anti-PLA1A antibody A09234. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-28
Question
Will anti-PLA1A antibody A09234 work on zebrafish WB with corpus epididymis?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not tested anti-PLA1A antibody A09234 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PLA1A antibody A09234 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish corpus epididymis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
We are currently using anti-PLA1A antibody A09234 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
S. Singh
Verified customer
Asked: 2016-11-15
Answer
The anti-PLA1A antibody (A09234) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-11-15