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Product Info Summary
| SKU: | A07219-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-D54 TPD52L2 Antibody
View all TPD52L2/D54 Antibodies
SKU/Catalog Number
A07219-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-D54 TPD52L2 Antibody catalog # A07219-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-D54 TPD52L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07219-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TPD54. AA range:141-190
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07219-1 is reactive to TPD52L2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
22238 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07219-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using D54 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TPD52L2 (Source: Uniprot.org, NCBI)
Gene Name
TPD52L2
Full Name
Tumor protein D54
Weight
22238 MW
Superfamily
TPD52 family
Alternative Names
Tumor protein D54;hD54;Tumor protein D52-like 2;TPD52L2; TPD52L2 D54, TPD54 TPD52 like 2 tumor protein D54|HCCR-binding protein 2|tumor protein D52 like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPD52L2, check out the TPD52L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPD52L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-D54 TPD52L2 Antibody (A07219-1)
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No publications found for A07219-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-D54 TPD52L2 Antibody
Question
I have a question about product A07219-1, anti-D54 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07219-1 anti-D54 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
I see that the anti-D54 antibody A07219-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-22
Question
Will anti-D54 antibody A07219-1 work on primate WB with placenta retinoblastoma?
J. Kulkarni
Verified customer
Asked: 2019-05-02
Answer
Our lab technicians have not tested anti-D54 antibody A07219-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-D54 antibody A07219-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta retinoblastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-02
Question
We want to test anti-D54 antibody A07219-1 on rat cervix carcinoma for research purposes, then I may be interested in using anti-D54 antibody A07219-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Krishna
Verified customer
Asked: 2018-08-22
Answer
The products we sell, including anti-D54 antibody A07219-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-22
Question
Will A07219-1 anti-D54 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
You can see on the product datasheet, A07219-1 anti-D54 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-18
Question
We are currently using anti-D54 antibody A07219-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
P. Roberts
Verified customer
Asked: 2017-04-25
Answer
The anti-D54 antibody (A07219-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-25
