Anti-D54 TPD52L2 Antibody

TPD52L2/D54 antibody

Boster Bio Anti-D54 TPD52L2 Antibody catalog # A07219-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07219-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-D54 TPD52L2 Antibody

View all TPD52L2/D54 Antibodies

SKU/Catalog Number

A07219-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-D54 TPD52L2 Antibody catalog # A07219-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-D54 TPD52L2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07219-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human D54. at AA rangle: 110-190

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07219-1 is reactive to TPD52L2 in Human, Mouse, Rat

Applications

A07219-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

22238 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For TPD52L2 (Source: Uniprot.org, NCBI)

Gene Name

TPD52L2

Full Name

Tumor protein D54

Weight

22238 MW

Superfamily

TPD52 family

Alternative Names

DKFZp686A1765; HCCR-binding protein 2; tumor protein D52-like 2hD54D54; tumor protein D54 TPD52L2 D54, TPD54 TPD52 like 2 tumor protein D54|HCCR-binding protein 2|tumor protein D52 like 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TPD52L2, check out the TPD52L2 Infographic

TPD52L2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TPD52L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-D54 TPD52L2 Antibody

Question

I have a question about product A07219-1, anti-D54 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07219-1 anti-D54 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-28

Question

I see that the anti-D54 antibody A07219-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-22

Question

Will anti-D54 antibody A07219-1 work on primate WB with placenta retinoblastoma?

J. Kulkarni

Verified customer

Asked: 2019-05-02

Answer

Our lab technicians have not tested anti-D54 antibody A07219-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-D54 antibody A07219-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta retinoblastoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-02

Question

We want to test anti-D54 antibody A07219-1 on rat cervix carcinoma for research purposes, then I may be interested in using anti-D54 antibody A07219-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Krishna

Verified customer

Asked: 2018-08-22

Answer

The products we sell, including anti-D54 antibody A07219-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-22

Question

Will A07219-1 anti-D54 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-07-18

Answer

You can see on the product datasheet, A07219-1 anti-D54 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-07-18

Question

We are currently using anti-D54 antibody A07219-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

P. Roberts

Verified customer

Asked: 2017-04-25

Answer

The anti-D54 antibody (A07219-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-04-25

Order DetailsPrice
A07219-1

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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