Product Info Summary
SKU: | A00247 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ARID1A Antibody
SKU/Catalog Number
A00247
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ARID1A Antibody (Catalog # A00247). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ARID1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00247)
Host
Rabbit
Contents
ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
Reactive Species
A00247 is reactive to ARID1A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
242045 MW
Background of ARID1A
The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00247 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of ARID1A in mouse brain tissue with ARID1A antibody at 5 μg/mL.
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Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
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Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ARID1A (Source: Uniprot.org, NCBI)
Gene Name
ARID1A
Full Name
AT-rich interactive domain-containing protein 1A
Weight
242045 MW
Alternative Names
AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1 ARID1A B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1 AT-rich interaction domain 1A AT-rich interactive domain-containing protein 1A|ARID domain-containing protein 1A|AT rich interactive domain 1A (SWI-like)|BRG1-associated factor 250a|OSA1 nuclear protein|SWI-like protein|SWI/SNF complex protein p270|SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1|brain protein 120|chromatin remodeling factor p250|osa homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARID1A, check out the ARID1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARID1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARID1A Antibody (A00247)
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