Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody

Product Name

Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody

View all Cytokeratin, HMW Antibodies

SKU/Catalog Number

M12092-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody (Catalog # M12092-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12092-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM116

Isotype

IgG1, kappa

Immunogen

Human epidermal keratin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M12092-1 is reactive to KRT76 in Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

65841 MW

Background of Cytokeratin, HMW

This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. This monoclonal antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M12092-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml f)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For KRT76 (Source: Uniprot.org, NCBI)

Alternative Names

Keratin, type II cytoskeletal 2 oral;Cytokeratin-2P;CK-2P;K2P;Keratin-76;K76;Type-II keratin Kb9;KRT76;KRT2B, KRT2P; KRT76 HUMCYT2A, KRT2B, KRT2P keratin 76 keratin, type II cytoskeletal 2 oral|CK-2P|K2P|K76|cytokeratin 2|cytokeratin-2P|keratin 2p|keratin 76, type II|type-II keratin Kb9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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