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Product Info Summary
| SKU: | M02101-4 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364
View all Cytokeratin 19 Antibodies
SKU/Catalog Number
M02101-4
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364 (Catalog # M02101-4). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02101-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM364
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CK-19 (Cytokeratin-19)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts human CK-19 (Cytokeratin-19)
Reactive Species
M02101-4 is reactive to KRT19 in Human
Applications
M02101-4 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44106 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of MCF-7 cell lysate using anti-CK-19 rabbit monoclonal antibody (Clone RM364) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-CK-19 rabbit monoclonal antibody (Clone RM364) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For KRT19 (Source: Uniprot.org, NCBI)
Gene Name
KRT19
Full Name
Keratin, type I cytoskeletal 19
Weight
44106 MW
Superfamily
intermediate filament family
Alternative Names
40-kDa keratin intermediate filament; CK19; CK-19; Cytokeratin 19; EndoC; K19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366 KRT19 CK19, K19, K1CS keratin 19 keratin, type I cytoskeletal 19|40-kDa keratin intermediate filament|CK-19|cytokeratin 19|keratin 19, type I|keratin, type I, 40-kd
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT19, check out the KRT19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364 (M02101-4)
Hello CJ!
M02101-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Cytokeratin 19 (CK-19) KRT19 Rabbit Monoclonal Antibody, Clone#RM364
Question
I would like using your anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 for keratinization studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
Thank you for your inquiry. This M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 is tested on breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-27
Question
Is a blocking peptide available for product anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 (M02101-4)?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do provide the blocking peptide for product anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 (M02101-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-16
Question
Will anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 work for WB with mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to the expression profile of mammary gland, KRT19 is highly expressed in mammary gland. So, it is likely that anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 will work for WB with mammary gland.
Boster Scientific Support
Answered: 2019-10-28
Question
Is this M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 reactive to the isotypes of KRT19?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The immunogen of M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 is A peptide corresponding to the C-terminus of human CK-19 (Cytokeratin-19). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-28
Question
We have observed staining in human pancreas placenta. Do you have any suggestions? Is anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 supposed to stain pancreas placenta positively?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
From literature pancreas placenta does express KRT19. From Uniprot.org, KRT19 is expressed in lower esophagus mucosa, placenta, mammary gland, pancreas placenta, peripheral blood leukocyte, keratinocyte, lymph node, cervix carcinoma erythroleukemia, colon carcinoma, among other tissues. Regarding which tissues have KRT19 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Keratinocyte, Pubmed ID: 1286667
Lymph node, Pubmed ID: 10212274
Mammary gland, Pancreas, and Placenta, Pubmed ID: 15489334
Peripheral blood leukocyte, Pubmed ID: 11682035
Placenta, Pubmed ID: 2447559, 2469734, 14702039
Boster Scientific Support
Answered: 2019-08-15
Question
I was wanting to use your anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 for WB for human mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human mammary gland identification?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
It shows on the product datasheet, M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-05
Question
We bought anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
I took a look at the website and datasheets of our anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-12-21
Question
Would M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Li
Verified customer
Asked: 2017-01-25
Answer
You can see on the product datasheet, M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-25
Question
I am interested in to test anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 on human mammary gland for research purposes, then I may be interested in using anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Anderson
Verified customer
Asked: 2016-03-10
Answer
The products we sell, including anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-10
Question
We are currently using anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
C. Miller
Verified customer
Asked: 2015-12-17
Answer
The anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 (M02101-4) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-17
Question
Is there a BSA free version of anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 available?
P. Li
Verified customer
Asked: 2015-10-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-10-30
Question
Can you help my question with product M02101-4, anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Jackson
Verified customer
Asked: 2014-03-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02101-4 anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-28
Question
I see that the anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4 works with WB, what is the protocol used to produce the result images on the product page?
S. Singh
Verified customer
Asked: 2013-08-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-02
Question
Please see the WB image, lot number and protocol we used for mammary gland using anti-Cytokeratin 19 (CK-19) Rabbit Monoclonal antibody, Clone#RM364 M02101-4. Please let me know if you require anything else.
C. Wu
Verified customer
Asked: 2013-03-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-11
