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Product Info Summary
| SKU: | M01357-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 18 KRT18 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01357-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 18 KRT18 Rabbit Monoclonal Antibody catalog # M01357-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 18 KRT18 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01357-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HB-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 18
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01357-1 is reactive to KRT18 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
48058 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01357-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
ICC/IF: HACAT cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Cytokeratin 18 expression in (1) HT-29 cell lysate;(2) human kidney lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HACAT cells, using Cytokeratin 18 Antibody .
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For KRT18 (Source: Uniprot.org, NCBI)
Gene Name
KRT18
Full Name
Keratin, type I cytoskeletal 18
Weight
48058 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type I cytoskeletal 18;Cell proliferation-inducing gene 46 protein;Cytokeratin-18;CK-18;Keratin-18;K18;KRT18;CYK18;PIG46; KRT18 CK-18, CYK18, K18 keratin 18 keratin, type I cytoskeletal 18|cell proliferation-inducing gene 46 protein|cytokeratin 18|keratin 18, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT18, check out the KRT18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 18 KRT18 Rabbit Monoclonal Antibody (M01357-1)
Hello CJ!
M01357-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cytokeratin 18 KRT18 Rabbit Monoclonal Antibody
Question
We ordered your anti-Cytokeratin 18 Rabbit Monoclonal antibody for IF on pancreas in the past. I am using human, and We are going to use the antibody for WB next. We want examining pancreas as well as adenohypophysis in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I looked at the website and datasheets of our anti-Cytokeratin 18 Rabbit Monoclonal antibody and it seems that M01357-1 has been validated on human in both IF and WB. Thus M01357-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-01
Question
Would M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-12
Question
Does anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to the expression profile of cervix carcinoma, KRT18 is highly expressed in cervix carcinoma. So, it is likely that anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2020-02-21
Question
I see that the anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-20
Question
I was wanting to use your anti-Cytokeratin 18 Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It shows on the product datasheet, M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
Is a blocking peptide available for product anti-Cytokeratin 18 Rabbit Monoclonal antibody (M01357-1)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We do provide the blocking peptide for product anti-Cytokeratin 18 Rabbit Monoclonal antibody (M01357-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-28
Question
Do you have a BSA free version of anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 available?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-26
Question
I am interested in to test anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
The products we sell, including anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-04
Question
My question regarding product M01357-1, anti-Cytokeratin 18 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-31
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1. Please let me know if you require anything else.
Z. Dhar
Verified customer
Asked: 2017-11-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1. Let me know if you need anything else.
K. Thomas
Verified customer
Asked: 2017-05-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-03
Question
Is this M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody reactive to the isotypes of KRT18?
A. Patel
Verified customer
Asked: 2017-04-04
Answer
The immunogen of M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytokeratin 18. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-04
Question
We have observed staining in human liver. Any tips? Is anti-Cytokeratin 18 Rabbit Monoclonal antibody supposed to stain liver positively?
C. Moore
Verified customer
Asked: 2016-09-13
Answer
Based on literature liver does express KRT18. Based on Uniprot.org, KRT18 is expressed in adenohypophysis, placenta, cervix, colon, pancreas, placenta uterus, vulva, liver, colon carcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381
Boster Scientific Support
Answered: 2016-09-13
Question
My lab would like using your anti-Cytokeratin 18 Rabbit Monoclonal antibody for extrinsic apoptotic signaling pathway studies. Has this antibody been tested with western blotting on hacat cells? We would like to see some validation images before ordering.
K. Li
Verified customer
Asked: 2016-03-23
Answer
We appreciate your inquiry. This M01357-1 anti-Cytokeratin 18 Rabbit Monoclonal antibody is validated on hacat cells. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-03-23
Question
We are currently using anti-Cytokeratin 18 Rabbit Monoclonal antibody M01357-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
G. Carter
Verified customer
Asked: 2014-05-14
Answer
The anti-Cytokeratin 18 Rabbit Monoclonal antibody (M01357-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-14
Question
My boss were content with the WB result of your anti-Cytokeratin 18 Rabbit Monoclonal antibody. However we have observed positive staining in placenta uterus perinuclear region. nucleus, using this antibody. Is that expected? Could you tell me where is KRT18 supposed to be expressed?
R. Dhar
Verified customer
Asked: 2013-10-09
Answer
From literature, placenta uterus does express KRT18. Generally KRT18 expresses in cytoplasm, perinuclear region. nucleus,. Regarding which tissues have KRT18 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 17924679, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Colon, Pancreas, Placenta, and Uterus, Pubmed ID: 15489334
Colon carcinoma, Pubmed ID: 9150948, 24129315
Liver, Pubmed ID: 2422083, 24275569
Placenta, Pubmed ID: 2434380
Vulva, Pubmed ID: 2434381
Boster Scientific Support
Answered: 2013-10-09
