Anti-Cytoglobin/CYGB Antibody Picoband®

Product Name

Anti-Cytoglobin/CYGB Antibody Picoband®

View all Cytoglobin Antibodies

SKU/Catalog Number

PA1657

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytoglobin/CYGB Antibody catalog # PA1657. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytoglobin/CYGB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1657)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin, identical to the related rat sequence, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1657 is reactive to CYGB in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

21 kDa

Calculated molecular weight

21405 MW

Background of Cytoglobin

Cytoglobin (CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir by Sawai et al.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1657 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Brain Tissue, Rat Small Intestine Tissue, Rat Liver Tissue, Rat Kidney Tissue, SGC Whole Cell, COLO320 Whole Cell, SMMC Whole Cell, PANC Whole Cell, HELA Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For CYGB (Source: Uniprot.org, NCBI)

Alternative Names

Cytoglobin;Histoglobin;HGb;Stellate cell activation-associated protein;CYGB;STAP; CYGB HGB, STAP cytoglobin cytoglobin|histoglobin|stellate cell activation-associated protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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