Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband™

Product Name

Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband™

View all Cytochrome P450 2A6 Antibodies

SKU/Catalog Number

A00947-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband™ catalog # A00947-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome P450 2A6/CYP2A6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00947-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2A6/CYP2A6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00947-2 is reactive to CYP2A6 in Human, Mouse, Rat

Applications

A00947-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

50408 MW

Background of Cytochrome P450 2A6

Cytochrome P450 2A6 (abbreviated CYP2A6) is a member of the cytochrome P450 mixed-function oxidase system, which is involved in the metabolism of xenobiotics in the body. CYP2A6 is the primary enzyme responsible for the oxidation of nicotine and cotinine. It is also involved in the metabolism of several pharmaceuticals, carcinogens, and a number of coumarin-type alkaloids. CYP2A6 is the only enzyme in the human body that appreciably catalyzes the 7-hydroxylation of coumarin, such that the formation of the product of this reaction, 7-hydroxycoumarin, is used as a probe for CYP2A6 activity. The CYP2A6 gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CYP2A6 using anti-CYP2A6 antibody (A00947-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat RH35 whole cell lysates,
Lane 2: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2A6 antigen affinity purified polyclonal antibody (Catalog # A00947-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2A6 at approximately 57KD. The expected band size for CYP2A6 is at 57KD.

Gene/Protein Information For CYP2A6 (Source: Uniprot.org, NCBI)

Alternative Names

Coumarin 7-hydroxylase; CPA6; CYP2A3P450C2A; CYP2AEC 1.14.14.1; CYPIIA6; cytochrome P450 2A6; Cytochrome P450 IIA3; Cytochrome P450(I); cytochrome P450, family 2, subfamily A, polypeptide 6; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6; flavoprotein-linked monooxygenase; P450PB; xenobiotic monooxygenase CYP2A6 CPA6, CYP2A, CYP2A3, CYPIIA6, P450C2A, P450PB cytochrome P450 family 2 subfamily A member 6 cytochrome P450 2A6|1,4-cineole 2-exo-monooxygenase|coumarin 7-hydroxylase|cytochrome P450 IIA3|cytochrome P450(I)|cytochrome P450, family 2, subfamily A, polypeptide 6|cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6|flavoprotein-linked monooxygenase|xenobiotic monooxygenase

For more info on CYP2A6, check out the CYP2A6 Infographic

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In this infographic, you will see the following information for CYP2A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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