Anti-Phospho-APC6 T580 CDC16 Antibody

Product Name

Anti-Phospho-APC6 T580 CDC16 Antibody

View all CDC16 Antibodies

SKU/Catalog Number

P04573

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-APC6 T580 CDC16 Antibody (Catalog # P04573). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-APC6 T580 CDC16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04573)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04573 is reactive to CDC16 in Human

Applications

P04573 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

71656 MW

Background of CDC16

APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. The APC/C is composed of at least 11 subunits. APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. Splice variants have been described for this protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:10,000
IF Microscopy: User optimized
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of phosphorylated APC6 expression in nocodazole treated HeLa whole cell lysate (lane 1) and lysates from asynchronous HeLa whole cell cultures (lane 2). Phosphorylated APC6 at 72KD was detected using rabbit anti-APC6 pT580 Antigen Affinity purified polyclonal antibody (Catalog # P04573) at 1:1000.

Gene/Protein Information For CDC16 (Source: Uniprot.org, NCBI)

Alternative Names

ANAPC6subunit 6; CDC16 (cell division cycle 16, S. cerevisiae, homolog); CDC16 cell division cycle 16 homolog (S. cerevisiae); CDC16 homolog; CDC16Hs; cell division cycle 16 homolog (S. cerevisiae); cell division cycle protein 16 homolog; Cyclosome subunit 6 CDC16 ANAPC6, APC6Hs, CUT9, CDC16 cell division cycle 16 cell division cycle protein 16 homolog|anaphase-promoting complex, subunit 6|cell division cycle 16 homolog|cyclosome subunit 6

For more info on CDC16, check out the CDC16 Infographic

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In this infographic, you will see the following information for CDC16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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