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Product Info Summary
| SKU: | M03529-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody
View all Cytochrome c Antibodies
SKU/Catalog Number
M03529-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody catalog # M03529-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FOA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome C
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03529-1 is reactive to CYCS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
11749 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03529-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human colonic cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of Cytochrome C expression in HeLa cell lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colonic cancer, using Cytochrome C Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CYCS (Source: Uniprot.org, NCBI)
Gene Name
CYCS
Full Name
Cytochrome c, somatic
Weight
11749 MW
Superfamily
cytochrome c family
Alternative Names
Cytochrome c;CYCS;CYC; Cycs|cytochrome c, somatic|cytochrome c, somatic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYCS, check out the CYCS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody (M03529-1)
Hello CJ!
M03529-1 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Z,Wang J,Zhu Y,Zhang H,Wang H.Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism.Mol Med Rep.2019 Nov;20(5):4612-4622.doi:10.3892/mmr.2019.10716.Epub 2019 Oct 1.PMID:31702040; PMCID:PMC6797977.
Species: Human,Rat
M03529-1 usage in article: APP:WB, SAMPLE:MITOCHONDRIAL TISSUE AND H9C2 CELL, DILUTION:1:1000
Zhang T,Chen Y,Cai J,Pan M,Sun Q,Zhang J,Sun C.SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes.Oxid Med Cell Longev.2020 Jul 13;2020:3742542.doi:10.1155/2020/3742542.PMID:32733634;PMCI
Species: Mouse
M03529-1 usage in article: APP:IF, SAMPLE:ADIPOCYTES, DILUTION:1:100
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Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
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N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody
Question
We are currently using anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 for mouse tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-Cytochrome C Rabbit Monoclonal antibody (M03529-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
My question regarding product M03529-1, anti-Cytochrome C Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
Does anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 work for WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
According to the expression profile of skeletal muscle, CYCS is highly expressed in skeletal muscle. So, it is likely that anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 will work for WB with skeletal muscle.
Boster Scientific Support
Answered: 2019-08-21
Question
I am interested in to test anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 on human skeletal muscle for research purposes, then I may be interested in using anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The products we sell, including anti-Cytochrome C Rabbit Monoclonal antibody M03529-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-19
Question
Is this M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody reactive to the isotypes of CYCS?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The immunogen of M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytochrome C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-05
Question
Would M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
As indicated on the product datasheet, M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
I see that the anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-23
Question
Please see the WB image, lot number and protocol we used for skeletal muscle using anti-Cytochrome C Rabbit Monoclonal antibody M03529-1. Please let me know if you require anything else.
P. Banerjee
Verified customer
Asked: 2018-07-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Cytochrome C Rabbit Monoclonal antibody M03529-1. Let me know if you need anything else.
C. Krishna
Verified customer
Asked: 2017-12-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-25
Question
We have observed staining in human amygdala. Are there any suggestions? Is anti-Cytochrome C Rabbit Monoclonal antibody supposed to stain amygdala positively?
R. Banerjee
Verified customer
Asked: 2017-01-23
Answer
Based on literature amygdala does express CYCS. Based on Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin,
rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-01-23
Question
Our lab were well pleased with the WB result of your anti-Cytochrome C Rabbit Monoclonal antibody. However we have observed positive staining in cerebellum mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?
A. Parker
Verified customer
Asked: 2016-12-06
Answer
From literature, cerebellum does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2016-12-06
Question
Is a blocking peptide available for product anti-Cytochrome C Rabbit Monoclonal antibody (M03529-1)?
B. Baker
Verified customer
Asked: 2016-07-25
Answer
We do provide the blocking peptide for product anti-Cytochrome C Rabbit Monoclonal antibody (M03529-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-25
Question
I was wanting to use your anti-Cytochrome C Rabbit Monoclonal antibody for WB for human skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human skeletal muscle identification?
V. Edwards
Verified customer
Asked: 2015-12-04
Answer
It shows on the product datasheet, M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-04
Question
Is there a BSA free version of anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 available?
R. Wu
Verified customer
Asked: 2015-04-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome C Rabbit Monoclonal antibody M03529-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-23
Question
We purchased anti-Cytochrome C Rabbit Monoclonal antibody for WB on skin, rc testis urinary bladder a few years ago. I am using human, and I plan to use the antibody for IP next. you antibody examining skin, rc testis urinary bladder as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
S. Carter
Verified customer
Asked: 2014-10-27
Answer
I looked at the website and datasheets of our anti-Cytochrome C Rabbit Monoclonal antibody and it seems that M03529-1 has been validated on human in both WB and IP. Thus M03529-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-10-27
Question
We want using your anti-Cytochrome C Rabbit Monoclonal antibody for protein dephosphorylation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
K. Mangal
Verified customer
Asked: 2013-12-20
Answer
Thank you for your inquiry. This M03529-1 anti-Cytochrome C Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-12-20
