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Product Info Summary
| SKU: | M03529-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody(monoclonal, 15F10)
View all Cytochrome c Antibodies
SKU/Catalog Number
M03529-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody (monoclonal, 15F10) catalog # M03529-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody(monoclonal, 15F10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529-Dyl550)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
15F10
Isotype
Mouse IgG1
Immunogen
E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03529-Dyl550 is reactive to CYCS in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
11.605kDa
Background of Cytochrome c
CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03529-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CYCS (Source: Uniprot.org, NCBI)
Gene Name
CYCS
Full Name
Cytochrome c, somatic
Weight
11.605kDa
Superfamily
cytochrome c family
Alternative Names
Cytochrome c; CYCS; CYC Cycs|cytochrome c, somatic|cytochrome c, somatic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYCS, check out the CYCS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody(monoclonal, 15F10) (M03529-Dyl550)
Hello CJ!
M03529-Dyl550 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pioglitazone improves mitochondrial function in the remnant kidney and protects against renal fibrosis in 5/6 nephrectomized rats
pH-sensitive micelles for the intracellular co-delivery of curcumin and Pluronic L61 unimers for synergistic reversal effect of multidrug resistance
Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells
Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway
Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells
Guo X, Fu Y, Wang Z, Wang T, Li C, Huang T, Gao F, Li C. Oxid Med Cell Longev. 2018 Mar 25;2018:4950705. doi: 10.1155/2018/4950705. eCollection 2018. Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition aga...
Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.
Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Cytochrome C DyLight® 550 conjugated CYCS Antibody(monoclonal, 15F10)
Question
I was wanting to use your anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) for Flow Cytometry for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
You can see on the product datasheet, M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-07
Question
We have seen staining in human amygdala. Any tips? Is anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) supposed to stain amygdala positively?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
According to literature amygdala does express CYCS. According to Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin,
rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-04-29
Question
Our team were content with the WB result of your anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10). However we have observed positive staining in erythroleukemia mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
From literature, erythroleukemia does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-02-27
Question
I am looking for to test anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 on human erythroleukemia for research purposes, then I may be interested in using anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
Will anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 work for Flow Cytometry with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
According to the expression profile of erythroleukemia, CYCS is highly expressed in erythroleukemia. So, it is likely that anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 will work for Flow Cytometry with erythroleukemia.
Boster Scientific Support
Answered: 2020-01-09
Question
I see that the anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Z. Yang
Verified customer
Asked: 2019-12-23
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-23
Question
Do you have a BSA free version of anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 available?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-03
Question
Can you help my question with product M03529-Dyl550, anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-20
Question
Is a blocking peptide available for product anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) (M03529-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We do provide the blocking peptide for product anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) (M03529-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-05
Question
Is this M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) reactive to the isotypes of CYCS?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The immunogen of M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) is E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-20
Question
We are currently using anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
T. Huang
Verified customer
Asked: 2019-05-17
Answer
The anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) (M03529-Dyl550) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550. Let me know if you need anything else.
K. Patel
Verified customer
Asked: 2019-01-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-22
Question
Would M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Parker
Verified customer
Asked: 2014-09-04
Answer
As indicated on the product datasheet, M03529-Dyl550 anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-04
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Human Cytochrome C DyLight® 550 conjugated antibody(monoclonal, 15F10) M03529-Dyl550. Please let me know if you require anything else.
A. Zhao
Verified customer
Asked: 2013-04-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-01
