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Product Info Summary
| SKU: | PB9564 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cystatin A/CSTA Antibody Picoband®
SKU/Catalog Number
PB9564
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cystatin A/CSTA Antibody Picoband® catalog # PB9564. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystatin A/CSTA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9564)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9564 is reactive to CSTA in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
11006 MW
Background of CSTA
Cystatin A also called Stefin A is a protein that in humans is encoded by the CSTA gene. Cystatin A is a cysteine proteinase inhibitor that belongs to family 1 of the cystatin superpamily. This gene was assigned to human chromosome 3q21.1. This is an intracellular thiol proteinase inhibitor. Cystatin A plays an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Stefins have been proposed as prognostic and diagnostic tools for cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9564 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human K562 whole cell
IHC: Human Tonsil tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PB9564).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody (Catalog # PB9564) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.
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Figure 2. IHC analysis of Cystatin A using anti-Cystatin A antibody (PB9564).
Cystatin A was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cystatin A Antibody (PB9564) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of Cystatin A using anti-Cystatin A antibody (PB9564).
Cystatin A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Cystatin A Antibody (PB9564) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CSTA (Source: Uniprot.org, NCBI)
Gene Name
CSTA
Full Name
Cystatin-A
Weight
11006 MW
Superfamily
cystatin family
Alternative Names
Cystatin-A;Cystatin-AS;Stefin-A;Cystatin-A, N-terminally processed;CSTA;STF1, STFA; CSTA AREI, PSS4, STF1, STFA cystatin A cystatin-A|cystatin A (stefin A)|cystatin AS
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSTA, check out the CSTA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSTA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystatin A/CSTA Antibody Picoband® (PB9564)
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Customer Q&As
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1 Customer Q&As for Anti-Cystatin A/CSTA Antibody Picoband®
Question
We are currently using anti-Cystatin A/CSTA antibody PB9564 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The anti-Cystatin A/CSTA antibody (PB9564) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-04
