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Product Info Summary
| SKU: | M00672 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYP2E1 Rabbit Monoclonal Antibody
View all Cytochrome P450 2E1 Antibodies
SKU/Catalog Number
M00672
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CYP2E1 Rabbit Monoclonal Antibody catalog # M00672. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP2E1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00672)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AADC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CYP2E1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00672 is reactive to CYP2E1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
56849 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00672 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CYP2E1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CYP2E1 (Source: Uniprot.org, NCBI)
Gene Name
CYP2E1
Full Name
Cytochrome P450 2E1
Weight
56849 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-J;Cytochrome P450 2E1, N-terminally processed;CYP2E1;CYP2E; CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2E1, check out the CYP2E1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP2E1 Rabbit Monoclonal Antibody (M00672)
Hello CJ!
M00672 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhai T,Xu W,Liu Y,Qian K,Xiong Y,Chen Y.Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway.Oxid Med Cell Longev.2020 Nov 27;2020:2313641.doi:10.1155/2020/231
Species: Mouse
M00672 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Schisandra sphenanthera extract (Wuzhi Tablet) protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway %u2026
Chen X, Sun Ck, Han Gz, Peng Jy, Li Y, Liu Yx, Lv Yy, Liu Kx, Zhou Q, Sun Hj. World J Gastroenterol. 2009 Apr 21;15(15):1829-35. Protective Effect Of Tea Polyphenols Against Paracetamol-Induced Hepatotoxicity In Mice Is Significantly Correlated Wi...
Fan X, Jiang Y, Wang Y, Tan H, Zeng H, Wang Y, Chen P, Qu A, Gonzalez Fj, Huang M, Bi H. Drug Metab Dispos. 2014 Dec;42(12):1982-90. Doi: 10.1124/Dmd.114.059535. Epub 2014 Sep 12. Wuzhi Tablet (Schisandra Sphenanthera Extract) Protects Against Ace...
Jiang Y, Fan X, Wang Y, Chen P, Zeng H, Tan H, Gonzalez Fj, Huang M, Bi H. Toxicol Sci. 2015 Jan;143(1):107-15. Doi: 10.1093/Toxsci/Kfu216. Epub 2014 Oct 14. Schisandrol B Protects Against Acetaminophen-Induced Hepatotoxicity By Inhibition Of Cyp-...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CYP2E1 Rabbit Monoclonal Antibody
Question
I was wanting to use to test anti-CYP2E1 Rabbit Monoclonal antibody M00672 on rat brain for research purposes, then I may be interested in using anti-CYP2E1 Rabbit Monoclonal antibody M00672 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The products we sell, including anti-CYP2E1 Rabbit Monoclonal antibody M00672, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CYP2E1 Rabbit Monoclonal antibody M00672. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-15
Question
Is this M00672 anti-CYP2E1 Rabbit Monoclonal antibody reactive to the isotypes of CYP2E1?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
The immunogen of M00672 anti-CYP2E1 Rabbit Monoclonal antibody is A synthesized peptide derived from human CYP2E1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-03
Question
My lab would like using your anti-CYP2E1 Rabbit Monoclonal antibody for response to organonitrogen compound studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We appreciate your inquiry. This M00672 anti-CYP2E1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-20
Question
We have seen staining in rat brain. What should we do? Is anti-CYP2E1 Rabbit Monoclonal antibody supposed to stain brain positively?
R. Parker
Verified customer
Asked: 2019-11-01
Answer
From literature brain does express CYP2E1. From Uniprot.org, CYP2E1 is expressed in liver, brain, among other tissues. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2019-11-01
Question
See below the WB image, lot number and protocol we used for brain using anti-CYP2E1 Rabbit Monoclonal antibody M00672. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
My question regarding product M00672, anti-CYP2E1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00672 anti-CYP2E1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-02
Question
Will anti-CYP2E1 Rabbit Monoclonal antibody M00672 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
According to the expression profile of brain, CYP2E1 is highly expressed in brain. So, it is likely that anti-CYP2E1 Rabbit Monoclonal antibody M00672 will work for WB with brain.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-CYP2E1 Rabbit Monoclonal antibody M00672 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The anti-CYP2E1 Rabbit Monoclonal antibody (M00672) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-CYP2E1 Rabbit Monoclonal antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
You can see on the product datasheet, M00672 anti-CYP2E1 Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
Question
I see that the anti-CYP2E1 Rabbit Monoclonal antibody M00672 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-30
Question
My boss were well pleased with the WB result of your anti-CYP2E1 Rabbit Monoclonal antibody. However we have observed positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP2E1 supposed to be expressed?
E. Brown
Verified customer
Asked: 2017-06-01
Answer
According to literature, liver does express CYP2E1. Generally CYP2E1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2017-06-01
Question
Would M00672 anti-CYP2E1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Collins
Verified customer
Asked: 2016-04-04
Answer
As indicated on the product datasheet, M00672 anti-CYP2E1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-04-04
Question
Is a blocking peptide available for product anti-CYP2E1 Rabbit Monoclonal antibody (M00672)?
J. Miller
Verified customer
Asked: 2015-10-16
Answer
We do provide the blocking peptide for product anti-CYP2E1 Rabbit Monoclonal antibody (M00672). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-10-16
Question
Is there a BSA free version of anti-CYP2E1 Rabbit Monoclonal antibody M00672 available?
G. Walker
Verified customer
Asked: 2013-01-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CYP2E1 Rabbit Monoclonal antibody M00672 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-01
