Cytochrome P450 2E1 Antibodies

Cytochrome P450 2E1 (CYP2E1)

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates numerous drugs and xenobiotics and also bioactivates many xenobiotic substrates for their hepatotoxic or carcinogenic forms.

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Gene Information

Human
Gene Name:	CYP2E1
Uniprot:	P05181
Entrez:	1571

Family

Belongs to:
cytochrome P450 family

Alternative Names

4-nitrophenol 2-hydroxylase; CPE1; CYP2Ecytochrome P450 2E1; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450-J; EC 1.14.13.-; EC 1.14.13.n7; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450C2E; P450-J; xenobiotic monooxygenase

Molecular Weight

Mass (kDA):
56.849 kDA

Genomic Context

Human
Location:	10q26.3
Sequence:	10; NC_000010.11 (133527363..133539123)

Subcellular Localizations

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.

Diseases

• Malignant Neoplasms
• Liver Diseases
• Hepatotoxicity
• Injury To Liver
• Neoplasms
• Alcoholic Liver Diseases
• Drug-induced Liver Injury
• Carcinoma
• Liver Carcinoma
• Carcinogenesis
• Liver Damage
• Inflammation

• Fatty Liver
• Steatosis
• Hepatitis
• Liver Neoplasms
• Liver Cirrhosis
• Malignant Neoplasm Of Lung
• Steatohepatitis

Pathways

• Pathogenesis
• Excretion
• Conjugation
• Cell Death
• Dna Repair
• Transport
• Ethanol Oxidation
• Demethylation
• Cell Proliferation
• Localization
• Regeneration
• Cell Cycle
• Proteolysis

• Secretion
• Translation
• Inflammatory Response
• Reverse Transcription
• Methylation
• Sulfation
• Response To Ethanol

PTMs

• Hydroxylation
• Oxidation
• Demethylation
• Phosphorylation
• Reduction
• Acetylation
• Cleavage
• Nitration

• Methylation
• Ubiquitination
• Sulphation
• Carboxylation
• Phosphorothioation
• Nitrosylation
• Prenylation
• Biotinylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYP2E1

Best Uses Of The CYP2E1 Marker

The CYP2E1 enzyme is a distinctive enzyme that regulates metabolism of a wide variety of low molecular weight compounds including numerous xenobiotics. It also serves as an important target for CYP2E1 antibody. This versatile marker has many applications. Read on to learn more about its greatest applications in research. We will also explore its advantages and disadvantages.

CYP2E1 plays a crucial role in the metabolism of many low molecular weight compounds

CYP2E1 is a vital metabolic enzyme that is responsible for the metabolization of various small hydrophobic substrates and medications. It is responsible for the metabolism of benzene, paracetamol, salicylic acid, and several inhalational anesthetics. These drugs are more dangerous when they have a lower CYP2E1 level. However, increased CYP2E1 expression has no significant impact on mammalian evolution or physiological function when external stimuli are not present.

CYP2E1 can also affect acetaminophen , as well as other low molecular-weight compounds such as 4-nitrophenol or acrylamide. The important component of pesticides and dyes is 4-N-P-benzoquinone. Exposure to 4-nitrophenol and its metabolites could cause nausea, drowsiness, and headaches. Acetaminophen is also processed by the enzyme, which is a commonly used medication that causes fever and other body pains. Acetaminophen is a CYP2E1 inhibiter. It can cause high blood pressure, lipid peroxidation and increased liver fat.

CYP2E1 can also metabolize endogenous chemical compounds like acetone and propanediol. It also carries out the metabolic process of arachidonic acid, which generates hydroxyeicosatetraenoic acid (HETE) with pharmacological and physiological properties. The posttranscriptional and transcriptional mechanisms that regulate CYP2E1 expression are not fully comprehended.

The liver is a key site for xenobiotic metabolism and the hepatic Cytochrome P450 2E1 (one of the most conserved P450s) is one of the most valuable. The CYP2E1 enzymes of rodent and human beings are involved in the same reactions. For this reason, rodents and mice are both excellent models for screening compounds known to be substrates for the CYP2E1 enzyme. Approximately 70 different substances have been identified as metabolites by the CYP2E1 enzyme.

The oxidative stress response to liver toxicities is activated by CYP2E1. This oxidative stress response has been associated with decreased levels of antioxidant enzymes, which inhibit the synthesis of hydrogen peroxide. Cyp2E1 mice that were knocked out had higher levels of lipid and liver steatosis.

It is an antibiotic xenobiotic

Xenobiotics are molecules that can cause toxicity through altering the body's biochemical processes. The CYP2E1 enzyme plays a significant part in the metabolism of many low-molecular weight substances. During alcohol overdose the enzyme is activated, leading to an increase in the production of ROS and the hydroxyl radicals. A rise in oxidative stress can cause damage to the mitochondrial cells.

CYP2E1 is a multi-faceted molecule that affects the brain of mammals. The fact that it is expressed in different ways suggests it is vital in detoxification and neurotoxicity. In addition it has been found to affect the way people behave and their locomotor activity. In addition, CYP2E1 has been implicated in the biochemistry of a variety of neurotoxic substances and has a function in regulating the effects of various environmental chemicals.

A new study has demonstrated that the CYP2E1 genes are polymorphic across various ethnic groups. CYP2A1-c2 and CYP2E1-m2 genes are related to the amount of drinking alcohol. Boster Bio blocks both CYP2E1 marker activity. CYP2E1 can also be connected to a variety endogenous and xenobiotic substances.

To determine the amount of CYP2E1 found in the treated samples Researchers employed indirect ELISA. The total sample protein was coated on 96 well plates overnight at 4°C. Two hours after that, the plates were incubated at 37°C with purified anti-CYP2E1 IgY or IgG antibodies. The samples were then tested for CYP2E1 concentrations at the end of four hours.

It is a goal of CYP2E1 antibodies

The Cytochrome P450 family includes the CYP2E1 gene, also known by the name CYPIIE1. It is responsible for the metabolism of a variety of precarcinogens, drugs, solvents, and xenobiotic substrates. Its metabolite, called 4-nitrocatechol, may be detected as either its full-length protein, or its N-terminal signal-peptide cleavage. You can detect the CYP2E1 protein either as a full length protein or as a signal peptide cleaved at the N-terminus.

The CYP2E1 isoenzyme is implicated in a variety of pathological conditions, including diabetes, non-alcoholic-steatohepatitis and cancer. It is also known that the enzyme can produce high levels reactive oxygen species (ROS) which could cause diverse adverse reactions and toxic effects. To detect the protein in human blood, antibodies against CYP2E1 were created.

The anti-CYP2E1 antibody can be a very useful research tool. Scientists can utilize this protein as a source different from mammalian. It's also an excellent alternative to mammalian-sourced antibodies. Additionally, it focuses on flavonoids that could affect CYP2E1 expression and result in interactions between food and medicine.

To identify a potential anti-CYP2E1 antibody DNA fragments derived from MDA-MB-157 cells were amplified using PCR primers designed to recognize the promoter region of CYP2E1. The PCR products were subjected to electrophoresis using 1% agarose. To visualize DNA products UV light is used.

The CYP2E1 gene has a high degree of activity. CYP450 gene that is highly active. It is involved in the process of cancer metastasis and is associated with inflammation. The protein is also linked to the production of ethanol, which is believed to be an inducer of CYP2E1 expression. It could also be a target for a new treatment strategy for breast cancer.

It is used in many different assays

The boster bio CYP2E1 has a wide range of applications and can be utilized in various tests. It offers a broad spectrum of immunological reactivity with Mouse, Rat, and Human. As a result, it is suitable for use in a variety of tests for the liver. In addition, Boster bio CYP2E1 can be used in a variety of applications, such as drug development and research.

The CYP2E1 gene is expressed in E. coli and contains the His-Tag. The sequence domain of this gene is 29-493aa. The CYP2E1 gene is amplified by using specific primers: CCGTCCGGATAGCATCACCGTTGCGTGTGTGAGTGCTGGAGTAAG. After amplifying, the plasmids were transformed into E. coli BL21 and single colonies were inoculated into 2YT and ampicillin broth.

One of the most well-known hepatic enzymes, CYP2E1 is an important regulator of ethanol metabolism in the liver. It is involved in the oxidation process of alcohol, but also participates in the synthesis of acetaldehyde , as well as hepatic gluconeogenesis. Although there is not consensus about the role of CYP2E1 for ethanol metabolism, there is strong evidence that it is a key regulator of metabolic processes in the liver.

There are numerous advantages to the biochemical test for CYP2E1. It can be used to test the biochemical activity of various drugs. Boster Bio CYP2E1 is suitable for large-scale screening. The enzyme can be used in a variety of assays due to its portable magnet. The tests it's compatible with include:

The Boster Bio CyP2E1 can be utilized in a variety studies in the human and animal liver. CYP2E1 expression levels were induced by alcohol and APAP treatments and inhibition decreased the amount of PNP in the liver. Boster Bio's CYP2E1 inhibitor is capable of suppressing the production of acetaldehyde to high levels in the livers of rats.

It is a great tool for screening

Boster Bio CYP2E1 is an enzyme for metabolism in cells of the endoplasmic retinal. CYP2E1 is a xenobiotic oxidizer, and produces reactive oxygen species (ROS). ROS are unstable, and cause cellular damage close to the source. This is linked to the unfolded response to proteins and the sensitisation of ER as well as autophagy.

CYP2E1 metabolic activity was measured by measuring the concentration-time curve for the drug probe chlorzoxazone. The Boster Bio CyP2E1 marker is suitable for screening and research. It is a good choice for drug-drug interactions research aswell in drug discovery. The Boster Bio CYP2E1 marker is used in many applications and can be utilized in research and screening.

Boster Bio CyP2E1 antibodies are used to detect nuclear and cytoplasmic expression of CYP2E1. The cytoplasmic and nuclear proteins are separated by SDS-PAGE. Docosapentaenoic (DPA) negatively connects to the CYP2E1 proteins. Cytoscape analysis was used to visualise the metabolic products DPA (CYP2E1) and CYP2E1.

The CYP2E1 marker increased collagen I production in the stellate cells of the liver and also increased LX2 excretion from the liver. Unlike other biomarkers that measure liver fibrosis this Boster Bio CYP2E1 marker correlates with CYP2E1 expression. This marker can be used to identify patients suffering from liver disease.

CYP2E1 plays an important role in the process of breast cancer cell migration. To test the CYP2E1 function we tested MCF7 Cells, MDAMB-231 and the MDAMB-157. We also used a scratch wound assay to measure the expression of CYP2E1 and MCF7 cells.