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Product Info Summary
| SKU: | M01316 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ADH1B Mouse Monoclonal Antibody [Clone ID: OTI4F12]
View all alcohol dehydrogenase 1B Antibodies
SKU/Catalog Number
M01316
Size
100 µl
Description
Boster Bio ADH1B mouse monoclonal antibody, clone OTI4F12 (formerly 4F12). Catalog# M01316. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ADH1B Mouse Monoclonal Antibody [Clone ID: OTI4F12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01316)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4F12
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human ADH1B (NP_000659) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01316 is reactive to ADH1B in Dog, Human, Rat
Reconstitution
Calculated molecular weight
39.855kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01316 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADH1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADH1B.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ADH1B monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ADH1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ADH1B (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADH1B antibody (M01316)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-ADH1B mouse monoclonal antibody (M01316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADH1B.
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Immunofluorescent staining of HeLa cells using anti-ADH1B mouse monoclonal antibody (M01316).
Protein Target Info & Infographic
Gene/Protein Information For ADH1B (Source: Uniprot.org, NCBI)
Gene Name
ADH1B
Full Name
All-trans-retinol dehydrogenase [NAD(+)] ADH1B
Weight
39.855kDa
Superfamily
zinc-containing alcohol dehydrogenase family
Alternative Names
ADH, beta subunit; ADH2alcohol dehydrogenase 1B; alcohol dehydrogenase 1B (class I), beta polypeptide; alcohol dehydrogenase 2 (class I), beta polypeptide; Alcohol dehydrogenase subunit beta; aldehyde reductase; DKFZp686C06125; EC 1.1.1; EC 1.1.1.1 ADH1B ADH2, HEL-S-117 alcohol dehydrogenase 1B (class I), beta polypeptide all-trans-retinol dehydrogenase [NAD(+)] ADH1B|ADH, beta subunit|alcohol dehydrogenase 2 (class I), beta polypeptide|alcohol dehydrogenase subunit beta|aldehyde reductase|epididymis secretory protein Li 117
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADH1B, check out the ADH1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADH1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADH1B Mouse Monoclonal Antibody [Clone ID: OTI4F12] (M01316)
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