Click image to see more details
Product Info Summary
| SKU: | PB9190 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
View all Cytochrome P450 2E1 Antibodies
SKU/Catalog Number
PB9190
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® catalog # PB9190. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9190)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9190 is reactive to CYP2E1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
56849 MW
Background of Cytochrome P450 2E1
Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9190 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat liver tissue, mouse liver tissue
IHC: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat liver tissue lysate,
lane 2: mouse liver tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # PB9190) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 55KD. The expected band size for CYP2E1 is at 57KD.
Click image to see more details
Figure 2. IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190).
CYP2E1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190).
CYP2E1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CYP2E1 (Source: Uniprot.org, NCBI)
Gene Name
CYP2E1
Full Name
Cytochrome P450 2E1
Weight
56849 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-J;Cytochrome P450 2E1, N-terminally processed;CYP2E1;CYP2E; CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2E1, check out the CYP2E1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (PB9190)
Hello CJ!
PB9190 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nephroprotective Effects of Anthocyanin from the Fruits of Panax ginseng (GFA) on Cisplatin‐Induced Acute Kidney Injury in Mice
Thida,M.,Li,B.,Zhang,X.,Chen,C.,& Zhang,X.(2021).Echinacoside alleviates acetaminophen-induced liver injury by attenuating oxidative stress and inflammatory cytokines in mice.J Appl Biomed,19(2),112.doi:10.32725/jab.2021.011
Species: Mouse
PB9190 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:500
Zhai T,Xu W,Liu Y,Qian K,Xiong Y,Chen Y.Honokiol Alleviates Methionine-Choline Deficient Diet-Induced Hepatic Steatosis and Oxidative Stress in C57BL/6 Mice by Regulating CFLAR-JNK Pathway.Oxid Med Cell Longev.2020 Nov 27;2020:2313641.doi:10.1155/2020/231
Species: Mouse
PB9190 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Schisandra sphenanthera extract (Wuzhi Tablet) protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway %u2026
Chen X, Sun Ck, Han Gz, Peng Jy, Li Y, Liu Yx, Lv Yy, Liu Kx, Zhou Q, Sun Hj. World J Gastroenterol. 2009 Apr 21;15(15):1829-35. Protective Effect Of Tea Polyphenols Against Paracetamol-Induced Hepatotoxicity In Mice Is Significantly Correlated Wi...
Fan X, Jiang Y, Wang Y, Tan H, Zeng H, Wang Y, Chen P, Qu A, Gonzalez Fj, Huang M, Bi H. Drug Metab Dispos. 2014 Dec;42(12):1982-90. Doi: 10.1124/Dmd.114.059535. Epub 2014 Sep 12. Wuzhi Tablet (Schisandra Sphenanthera Extract) Protects Against Ace...
Jiang Y, Fan X, Wang Y, Chen P, Zeng H, Tan H, Gonzalez Fj, Huang M, Bi H. Toxicol Sci. 2015 Jan;143(1):107-15. Doi: 10.1093/Toxsci/Kfu216. Epub 2014 Oct 14. Schisandrol B Protects Against Acetaminophen-Induced Hepatotoxicity By Inhibition Of Cyp-...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Cytochrome P450 2E1/CYP2E1 antibody (PB9190)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
We do provide the blocking peptide for product anti-Cytochrome P450 2E1/CYP2E1 antibody (PB9190). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-08
Question
We are currently using anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-Cytochrome P450 2E1/CYP2E1 antibody (PB9190) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
We purchased anti-Cytochrome P450 2E1/CYP2E1 antibody for WB on liver in a previous project. I am using mouse, and I plan to use the antibody for IHC next. We need examining liver as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
I have checked the website and datasheets of our anti-Cytochrome P450 2E1/CYP2E1 antibody and it seems that PB9190 has been tested on mouse in both WB and IHC. Thus PB9190 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
I was wanting to use your anti-Cytochrome P450 2E1/CYP2E1 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
As indicated on the product datasheet, PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody has been validated for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-06
Question
My question regarding product PB9190, anti-Cytochrome P450 2E1/CYP2E1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-04
Question
Will anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to the expression profile of liver, CYP2E1 is highly expressed in liver. So, it is likely that anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 will work for WB with liver.
Boster Scientific Support
Answered: 2019-12-25
Question
We have seen staining in rat brain. Are there any suggestions? Is anti-Cytochrome P450 2E1/CYP2E1 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
From what I have seen in literature brain does express CYP2E1. From what I have seen in Uniprot.org, CYP2E1 is expressed in liver, brain, among other tissues. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2019-11-29
Question
Will PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
As indicated on the product datasheet, PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-01
Question
See attached the WB image, lot number and protocol we used for liver using anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-11
Question
We are interested in using your anti-Cytochrome P450 2E1/CYP2E1 antibody for benzene metabolic process studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
I appreciate your inquiry. This PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody is tested on rat liver tissue, tissue lysate, mouse liver tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-05
Question
Is there a BSA free version of anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 available?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-07
Question
My team were happy with the WB result of your anti-Cytochrome P450 2E1/CYP2E1 antibody. However we have seen positive staining in liver endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP2E1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
According to literature, liver does express CYP2E1. Generally CYP2E1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP2E1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 3675576, 24275569
Boster Scientific Support
Answered: 2017-10-30
Question
Is this PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody reactive to the isotypes of CYP2E1?
P. Jha
Verified customer
Asked: 2015-11-25
Answer
The immunogen of PB9190 anti-Cytochrome P450 2E1/CYP2E1 antibody is E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-11-25
Question
I see that the anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 works with WB, what is the protocol used to produce the result images on the product page?
P. Wu
Verified customer
Asked: 2015-10-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-10-14
Question
you antibody to test anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 on mouse liver for research purposes, then I may be interested in using anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Krishna
Verified customer
Asked: 2013-07-01
Answer
The products we sell, including anti-Cytochrome P450 2E1/CYP2E1 antibody PB9190, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-01
