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Product Info Summary
| SKU: | A00615 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CYP17A1 (N249) Antibody
View all Cytochrome P450 17A1 Antibodies
SKU/Catalog Number
A00615
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CYP17A1 (N249) Antibody catalog # A00615. Tested in WB,IHC,IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP17A1 (N249) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00615)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 211-260 of Human CYP17A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00615 is reactive to CYP17A1 in Human
Reconstitution
Calculated molecular weight
57371 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00615 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of CYP17A1 using Anti-CYP17A1 (N249) Antibody (A00615).
Immunohistochemistry (IHC) analyzes of CYP17A1 (N249) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-CYP17A1 (N249) Antibody A00615
Western blot (WB) analysis of CYP17A1 (N249) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate(40ug)
Lane2:A2780 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYP17A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP17A1
Full Name
Steroid 17-alpha-hydroxylase/17,20 lyase
Weight
57371 MW
Superfamily
cytochrome P450 family
Alternative Names
Steroid 17-alpha-hydroxylase/17,20 lyase;1.14.14.19 CYP17A1 CPT7, CYP17, P450C17, S17AH cytochrome P450 family 17 subfamily A member 1 steroid 17-alpha-hydroxylase/17,20 lyase|17-alpha-hydroxyprogesterone aldolase|CYPXVII|cytochrome P450 17A1|cytochrome P450, family 17, subfamily A, polypeptide 1|cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia|cytochrome P450-C17|cytochrome P450c17|cytochrome p450 XVIIA1|steroid 17-alpha-monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP17A1, check out the CYP17A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP17A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP17A1 (N249) Antibody (A00615)
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