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Product Info Summary
| SKU: | PA1698 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CYP11A1 Antibody Picoband®
SKU/Catalog Number
PA1698
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP11A1 Antibody catalog # PA1698. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP11A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1698)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1698 is reactive to CYP11A1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
60102 MW
Background of CYP11A1
CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1698 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue
IHC: Human Placenta Tissue
IHC-F: Human Placenta Tissue
Validation Images & Assay Conditions
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Anti-CYP11A1 antibody, PA1698, Western blotting
WB: Human Placenta Tissue Lysate
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Anti-CYP11A1 antibody, PA1698, IHC(P)
IHC(P): Human Placenta Tissue
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Anti-CYP11A1 antibody, PA1698, IHC(F)
IHC(F): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For CYP11A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP11A1
Full Name
Cholesterol side-chain cleavage enzyme, mitochondrial
Weight
60102 MW
Superfamily
cytochrome P450 family
Alternative Names
Cholesterol side-chain cleavage enzyme, mitochondrial;1.14.15.6;CYPXIA1;Cholesterol desmolase;Cytochrome P450 11A1;Cytochrome P450 (scc);CYP11A1;CYP11A; CYP11A1 CYP11A, CYPXIA1, P450SCC cytochrome P450 family 11 subfamily A member 1 cholesterol side-chain cleavage enzyme, mitochondrial|cholesterol 20-22 desmolase|cholesterol monooxygenase (side-chain cleaving)|cytochrome P450 11A1|cytochrome P450 family 11 subfamily A polypeptide 1|cytochrome P450(scc)|cytochrome P450, subfamily XIA (cholesterol side chain cleavage)|steroid 20-22-lyase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP11A1, check out the CYP11A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP11A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP11A1 Antibody Picoband® (PA1698)
Hello CJ!
PA1698 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Abnormal Apoptosis of Trophoblastic Cells Is Related to the Up-Regulation of CYP11A Gene in Placenta of Preeclampsia Patients
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CYP11A1 Antibody Picoband®
Question
We are currently using anti-CYP11A1 antibody PA1698 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
The anti-CYP11A1 antibody (PA1698) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-12
Question
We have seen staining in human placenta. Are there any suggestions? Is anti-CYP11A1 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
According to literature placenta does express CYP11A1. According to Uniprot.org, CYP11A1 is expressed in right adrenal gland, placenta, placenta testis, brain, choriocarcinoma, among other tissues. Regarding which tissues have CYP11A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Choriocarcinoma, Pubmed ID: 1849407
Placenta, Pubmed ID: 3038854
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-22
Question
I would like using your anti-CYP11A1 antibody for congenital studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We appreciate your inquiry. This PA1698 anti-CYP11A1 antibody is tested on human placenta tissue, tissue lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-17
Question
My colleagues were content with the WB result of your anti-CYP11A1 antibody. However we have been able to see positive staining in placenta testis mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is CYP11A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
From what I have seen in literature, placenta testis does express CYP11A1. Generally CYP11A1 expresses in mitochondrion inner membrane. Regarding which tissues have CYP11A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Choriocarcinoma, Pubmed ID: 1849407
Placenta, Pubmed ID: 3038854
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-30
Question
We purchased anti-CYP11A1 antibody for WB on placenta testis in a previous project. I am using human, and I plan to use the antibody for IHC next. My question regards examining placenta testis as well as choriocarcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
V. Anderson
Verified customer
Asked: 2015-12-22
Answer
I viewed the website and datasheets of our anti-CYP11A1 antibody and it appears that PA1698 has been tested on human in both WB and IHC. Thus PA1698 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-12-22
