Anti-CYP11A1 Antibody

Product Name

Anti-CYP11A1 Antibody

View all CYP11A1 Antibodies

SKU/Catalog Number

PA1698

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CYP11A1 Antibody catalog # PA1698. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYP11A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1698)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1698 is reactive to CYP11A1 in Human

Applications

PA1698 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

60102 MW

Background of CYP11A1

CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-CYP11A1 antibody, PA1698, Western blotting
WB: Human Placenta Tissue Lysate

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Anti-CYP11A1 antibody, PA1698, IHC(P)
IHC(P): Human Placenta Tissue

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Anti-CYP11A1 antibody, PA1698, IHC(F)
IHC(F): Human Placenta Tissue

Gene/Protein Information For CYP11A1 (Source: Uniprot.org, NCBI)

Alternative Names

Cholesterol desmolase; cholesterol monooxygenase (side-chain-cleaving); cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450(scc); cytochrome P450, family 11, subfamily A, polypeptide 1; cytochrome P450C11A1; EC 1.14.15; EC 1.14.15.6; steroid 20-22-lyase; subfamily XIA (cholesterol side chain cleavage) CYP11A1 CYP11A, CYPXIA1, P450SCC cytochrome P450 family 11 subfamily A member 1 cholesterol side-chain cleavage enzyme, mitochondrial|cholesterol 20-22 desmolase|cholesterol monooxygenase (side-chain cleaving)|cytochrome P450 11A1|cytochrome P450 family 11 subfamily A polypeptide 1|cytochrome P450(scc)|cytochrome P450, subfamily XIA (cholesterol side chain cleavage)|steroid 20-22-lyase

For more info on CYP11A1, check out the CYP11A1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP11A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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