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Product Info Summary
| SKU: | M00543-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin E1/CCNE1 Antibody Picoband® (monoclonal, 8B9C3)
SKU/Catalog Number
M00543-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin E1/CCNE1 Antibody Picoband® (monoclonal, 8B9C3) catalog # M00543-3. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cyclin E1/CCNE1 Antibody Picoband® (monoclonal, 8B9C3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00543-3)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8B9C3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Cyclin E1/CCNE1 recombinant protein (Position: R3-A386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00543-3 is reactive to CCNE1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.077kDa
Background of Cyclin E1
G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00543-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human K562 whole cell, human A549 whole cell, human CACO-2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CCNE1 using anti-CCNE1 antibody (M00543-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CCNE1 antigen affinity purified monoclonal antibody (Catalog # M00543-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47 kDa. The expected band size for CCNE1 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CCNE1 (Source: Uniprot.org, NCBI)
Gene Name
CCNE1
Full Name
G1/S-specific cyclin-E1
Weight
47.077kDa
Superfamily
cyclin family
Alternative Names
Serum paraoxonase/arylesterase 1; PON 1 CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNE1, check out the CCNE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin E1/CCNE1 Antibody Picoband® (monoclonal, 8B9C3) (M00543-3)
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