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Product Info Summary
| SKU: | A00543-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cyclin E1 CCNE1 Antibody
SKU/Catalog Number
A00543-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cyclin E1 CCNE1 Antibody (Catalog # A00543-1). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin E1 CCNE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00543-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cyclin E1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00543-1 is reactive to CCNE1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
47077 MW
Background of Cyclin E1
Essential for the control of the cell cycle at the G1/S (start) transition.
Charles Spruck, Cancer Res., Jul 2006; 66: 7355 - 7360.
Nathalie Cueille, J. Virol., Sep 1998; 72: 7255 - 7262.
Wentao Deng, J. Virol., Dec 2004; 78: 13954 - 13965.
Tianlin Ma, PNAS, Jan 1999; 96: 382.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00543-1 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using Cyclin E1 antibody A00543-1.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin E1 antibody A00543-1.
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Western blot analysis of extracts from A549 cells (Lane 2), using Cyclin E1 antiobdy A00543-1. The lane on the left is treated with systhesized peptide.
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Immunofluorescence analysis of A549 cells, using Cyclin E1 antibody A00543-1.
Protein Target Info & Infographic
Gene/Protein Information For CCNE1 (Source: Uniprot.org, NCBI)
Gene Name
CCNE1
Full Name
G1/S-specific cyclin-E1
Weight
47077 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-E1;CCNE1;CCNE; CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNE1, check out the CCNE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin E1 CCNE1 Antibody (A00543-1)
Hello CJ!
A00543-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.
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