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Product Info Summary
| SKU: | A01744 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IHC |
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Product info
Product Name
Anti-Cyclin D3 Ccnd3 Antibody
SKU/Catalog Number
A01744
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cyclin D3 Ccnd3 Antibody (Catalog # A01744). Tested in ELISA, Flow Cytometry, IHC, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cyclin D3 Ccnd3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01744)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01744 is reactive to CCND3 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
46169 MW
Background of Cyclin D3
Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01744 is guaranteed for ELISA, Flow Cytometry, IP, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1000 - 1:5000
Flow Cytometry: 4µg/mL
IHC: 4µg/mL
IP: 5µg/mL
WB: 1.0 ug/ml
Validation Images & Assay Conditions
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Immunoprecipitation of Rabbit Anti-Cyclin D3.Lane 1: 5
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ELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1
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Flow Cytometry of Rabbit anti-Cyclin D3 antibody.Cells:mouse B cells+, 14 days post immunization (NP-CGG).Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.
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Figure 1. IHC analysis of Ccnd3 using anti-Ccnd3 antibody (A01744).
Ccnd3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ccnd3 Antibody (A01744) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CCND3 (Source: Uniprot.org, NCBI)
Gene Name
CCND3
Full Name
G1/S-specific cyclin-D3
Weight
46169 MW
Superfamily
cyclin family
Alternative Names
Cyclin D3, G1/S-specific cyclin-D3, Ccnd3, Cyl-3 CCND3 cyclin D3 G1/S-specific cyclin-D3|D3-type cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND3, check out the CCND3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D3 Ccnd3 Antibody (A01744)
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