Anti-DTX4 Antibody

Product Name

Anti-DTX4 Antibody

View all DTX4 Antibodies

SKU/Catalog Number

A12829

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DTX4 Antibody (Catalog # A12829). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-DTX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12829)

Host

Rabbit

Contents

DTX4 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4. The immunogen is located within amino acids 230 - 280 of DTX4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.

Reactive Species

A12829 is reactive to DTX4 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

67258 MW

Background of DTX4

The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12829 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DTX4 (Source: Uniprot.org, NCBI)

Alternative Names

Deltex 4 E3 ubiquitin ligase, RNF155 DTX4 RNF155 deltex E3 ubiquitin ligase 4 E3 ubiquitin-protein ligase DTX4|RING finger protein 155|RING-type E3 ubiquitin transferase DTX4|deltex 4 homolog|deltex 4, E3 ubiquitin ligase|deltex homolog 4|deltex4|protein deltex-4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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