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Product Info Summary
| SKU: | M00745-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00745-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody catalog # M00745-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EOG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin B1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00745-2 is reactive to CCNB1 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
133 kDa
Calculated molecular weight
48337 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00745-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
IHC: Human colon tissue, Human lung large cell cancer tissue, Rat stomach tissue, Mouse lung tissue, Human esophageal carcinoma tissue, human colon cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6k dilution for 1 hour at room temperature.
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Western blot analysis of Cyclin B1 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse lung, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin B1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using Cyclin B1 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48337 MW
Superfamily
cyclin family
Alternative Names
G2/mitotic-specific cyclin-B1;CCNB1;CCNB; CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (M00745-2)
Hello CJ!
M00745-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang H,Su Y,Wang J,Gao Y,Yang F,Li G,Shi Q.Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway.Life Sci.2019 Jul 15;229:210-218.doi:10.1016/j.lfs.2019.05.033.Epub 2019 May 16.PMID:31102746.
Species: American Minks
M00745-2 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:400
Nan W,Li G,Si H,Lou Y,Wang D,Guo R,Zhang H.All-trans-retinoic acid inhibits mink hair follicle growth via inhibiting proliferation and inducing apoptosis of dermal papilla cells through TGF-β2/Smad2/3 pathway. Acta Histochem. 2020 Oct;122(7):151603.doi: 1
Species: American mink
M00745-2 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:300
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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