Product Info Summary
SKU: | A03889-3 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cyclin A (W250) CCNA1 Antibody
SKU/Catalog Number
A03889-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cyclin A (W250) CCNA1 Antibody catalog # A03889-3. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin A (W250) CCNA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03889-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 220-270 of Human Cyclin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03889-3 is reactive to CCNA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
52358 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03889-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CCNA1 using Anti-Cyclin A (W250) CCNA1 Antibody (A03889-3).
Immunohistochemistry (IHC) analyzes of Cyclin A (W250) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Cyclin A (W250) CCNA1 Antibody A03889-3
Western blot (WB) analysis of Cyclin A (W250) pAb at 1:500 dilutionLane1:K562 whole cell lysate(40ug)
Lane2:SGC7901 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Mouse(40ug)
Lane4:The Testis tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CCNA1 (Source: Uniprot.org, NCBI)
Gene Name
CCNA1
Full Name
Cyclin-A1
Weight
52358 MW
Superfamily
cyclin family
Alternative Names
Cyclin-A1;CCNA1; CCNA1 CT146 cyclin A1 cyclin-A1|testicular tissue protein Li 34
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNA1, check out the CCNA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin A (W250) CCNA1 Antibody (A03889-3)
Hello CJ!
A03889-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chaowei Hu,Kun Zuo,Kuibao Li,Yuanfeng Gao,Mulei Chen,Roumu Hu,Ye Liu,Hongjie Chi,Hongjiang Wang,Yanwen Qin,Xiaoyan Liu,Jiuchang Zhong,Jun Cai,Xinchun Yang,Jing Li, "p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle
Species: Rat,Human
A03889-3 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
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