Product Info Summary
SKU: | PA2043 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PADI4/PAD4 Antibody
SKU/Catalog Number
PA2043
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PADI4/PAD4 Antibody catalog # PA2043. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PADI4/PAD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2043)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence in the middle region of human PADI4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2043 is reactive to Padi4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
74079 MW
Background of Padi4
PADI4 (Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion (Nakashima et al., 1999). Suzuki et al. (2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al. (2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2043 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: PANC Cell, 293T Cell,
Validation Images & Assay Conditions
![pa2043 1 WB anti padi4 pad4 antibody pa2043 1 WB anti padi4 pad4 antibody](https://www.bosterbio.com/media/catalog/product/antibody/pa2043-1-WB-anti-padi4-pad4-antibody.jpg)
Click image to see more details
Anti-PADI4/PAD4 antibody, PA2043, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: 293T Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Padi4 (Source: Uniprot.org, NCBI)
Gene Name
Padi4
Full Name
Protein-arginine deiminase type-4
Weight
74079 MW
Superfamily
protein arginine deiminase family
Alternative Names
HL-60 PAD; PAD; PAD4; PADI4; PADI5PADI-H protein; PADPAD4; PDI4; PDI5EC 3.5.3.15; peptidyl arginine deiminase, type IV; peptidyl arginine deiminase, type V; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV; protein-arginine deiminase type-4 Padi4|Pa, Pad4, Pd, Pdi4|peptidyl arginine deiminase, type IV|protein-arginine deiminase type-4|PAD type IV|peptidylarginine deiminase IV|protein-arginine deiminase type IV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Padi4, check out the Padi4 Infographic
![Padi4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Padi4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PADI4/PAD4 Antibody (PA2043)
Hello CJ!
No publications found for PA2043
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PADI4/PAD4 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PADI4/PAD4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-PADI4/PAD4 Antibody
Question
Here is the WB image, lot number and protocol we used for brain using anti-PADI4/PAD4 antibody PA2043. Please let me know if you require anything else.
G. Johnson
Verified customer
Asked: 2020-04-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-PADI4/PAD4 antibody PA2043 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The anti-PADI4/PAD4 antibody (PA2043) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-26
Question
Is a blocking peptide available for product anti-PADI4/PAD4 antibody (PA2043)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-PADI4/PAD4 antibody (PA2043). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-PADI4/PAD4 antibody PA2043 work on horse WB with blood?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
Our lab technicians have not validated anti-PADI4/PAD4 antibody PA2043 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PADI4/PAD4 antibody PA2043 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-09
Question
I was wanting to use your anti-PADI4/PAD4 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
A. Jha
Verified customer
Asked: 2015-07-09
Answer
As indicated on the product datasheet, PA2043 anti-PADI4/PAD4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-09