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Product Info Summary
| SKU: | A00700 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclin A CCNA2 Antibody
SKU/Catalog Number
A00700
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cyclin A CCNA2 Antibody (Catalog # A00700). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cyclin A CCNA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00700)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00700 is reactive to CCNA2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
48551 MW
Background of Cyclin A2
Anti-Cyclin A detects Cyclin A. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-Cyclin A Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00700 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:100
IP: 1:100
WB: 1:500 - 1:5,000
Validation Images & Assay Conditions
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Western blot analysis of Cyclin A expression in T98G cells treated with normal (n) (lane 1), T98G cells treated with scrambled (scr) (lane 2) and T98G cells treated with PIN1 knockdown (kd1) (lane 3). Cyclin A at 60KD was detected using rabbit anti-Cyclin A polyclonal antibody (Catalog # A00700) at 1:400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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Cyclin A was detected in paraffin-embedded sections of human testes tissues using rabbit anti-Cyclin A polyclonal antibody (Catalog # A00700) at 1:500. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For CCNA2 (Source: Uniprot.org, NCBI)
Gene Name
CCNA2
Full Name
Cyclin-A2
Weight
48551 MW
Superfamily
cyclin family
Alternative Names
Cyclin A, CCN1, CCNA, CCNA2, Cyclin A2 CCNA2 CCN1, CCNA cyclin A2 cyclin-A2|cyclin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNA2, check out the CCNA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin A CCNA2 Antibody (A00700)
Hello CJ!
A00700 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LncRNA HCP5 promotes cell proliferation and inhibits apoptosis via miR-27a-3p/IGF-1 axis in human granulosa-like tumor cell line KGN
Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
A00700 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200
Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z. Biomed Res Int. 2015;2015:304753. Doi: 10.1155/2015/304753. Epub 2015 Mar 30. A New Double Stranded Rna Suppresses Bladder Cancer Development By Upregulating P21 (Waf1/Cip1) Expression.
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