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Product Info Summary
| SKU: | EK1379 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse FGF21 ELISA Kit PicoKine®
SKU/Catalog Number
EK1379
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FGF21 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fgf21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FGF21 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1379)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A29-S210
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1379 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1379 is reactive to Fgf21 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1379 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF-21
Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1379-CAP | Anti-Mouse Fgf21 Pre-coated 96-well strip microplate | 1 |
| EK1379-ST | Mouse Fgf21 Standard | 2 vials, 10 ng/tube |
| EK1379-DA | Mouse Fgf21 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.074 | 0.134 | 0.248 | 0.484 | 0.803 | 1.355 | 1.936 |
Data Example Images
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Mouse FGF21 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF-21, Dilution ratio of 1:1, concentration in serum and plasma is 500-700 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FGF21 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 64 | 320 | 918 | 65 | 289 | 938 |
| Standard deviation | 2.81 | 24 | 52.32 | 3.12 | 27.16 | 70.35 |
| CV (%) | 4.4% | 7.5% | 5.7% | 4.8% | 9.4% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FGF-21 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 64 | 62 | 61 | 54 | 60 | 3.76 | 6.2% |
| Sample 2 | 320 | 285 | 310 | 296 | 302 | 13.32 | 4.4% |
| Sample 3 | 918 | 928 | 964 | 981 | 947 | 25.71 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For Fgf21 (Source: Uniprot.Org, NCBI)
Gene Name
Fgf21
Full Name
Fibroblast growth factor 21
Weight
23237 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 21;FGF-21;Fgf21; FGF21 fibroblast growth factor 21 fibroblast growth factor 21
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Fgf21, check out the Fgf21 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fgf21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FGF21 ELISA Kit PicoKine® (EK1379)
Hello CJ!
EK1379 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Role of Kupffer Cells as Mediators of Adipose Tissue Lipolysis
CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins
Liu M, Pan Q, Chen Y, Yang X, Zhao B, Jia L, Zhu Y, Zhang B, Gao X, Li X, Han J, Duan Y. Sci Rep. 2015 Jun 10;5:11219. Doi: 10.1038/Srep11219. Administration Of Danhong Injection To Diabetic Db/Db Mice Inhibits The Development Of Diabetic Retinopa...
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Customer Q&As
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5 Customer Q&As for Mouse FGF21 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Green
Verified customer
Asked: 2020-08-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-22
Question
Q: how do I analyze ELISA data? I have obtained FGF-21 level in serum.
K. Patel
Verified customer
Asked: 2019-11-03
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-03
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement FGF-21 using the EK1379 Mouse FGF-21 Picokine® ELISA Kit?
D. Varma
Verified customer
Asked: 2018-10-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-10-26
Question
We have a customer who wants to purchase your Human PROS1 PicoKine ELISA Kit EK1739-96WELLS because they are interested in the human PROS1. However, at a later time point maybe also PROS1 in mice. Do you have any data if the kit might work with mice? Normally you say if the sequence if the sequences are 90%+ identical, there is a high chance of cross-reactivity
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
Our lab technicians didn't detect PROS1 in serum from healthy mice. Best Regards,
Boster Scientific Support
Answered: 2017-06-08
Question
I only have 20 µL of plasma sample. Can I dilute the sample to 100 µL for this assay?
Verified Customer
Verified customer
Asked: 2014-08-14
Answer
Yes if the sample is mouse plasma. Note that we do not recommend this dilution for human plasma because its FGF21 level is lower than that in mouse plasma.
Boster Scientific Support
Answered: 2014-08-14
