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Product Info Summary
| SKU: | A09710 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, WB |
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Product info
Product Name
Anti-Cybr CYTIP Antibody
SKU/Catalog Number
A09710
Size
100uL
Form
Lyophilized
Description
Boster Bio Anti-Cybr CYTIP Antibody (Catalog # A09710). Tested in ELISA, IP, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store PSCDBP antibody / Anti-Cybr at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cybr CYTIP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09710)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09710 is reactive to CYTIP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
29277 MW
Background of CYTIP
Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells). Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09710 is guaranteed for ELISA, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IP: 1:100
WB: 1:1,000 - 1:10,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CYTIP using anti-CYTIP antibody (A09710).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYTIP antigen affinity purified polyclonal antibody (Catalog # A09710) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CYTIP.
Protein Target Info & Infographic
Gene/Protein Information For CYTIP (Source: Uniprot.org, NCBI)
Gene Name
CYTIP
Full Name
Cytohesin-interacting protein
Weight
29277 MW
Alternative Names
Cybr, Cytohesin binder and regulator, Cytohesin-binding protein HE, Cytohesin-interacting protein, Pleckstrin Homology Sec7 and Coiled-Coil Domains Binding Protein, B31, PSCDBP CYTIP B3-1, CASP, CYBR, CYTHIP, HE, PSCDBP cytohesin 1 interacting protein cytohesin-interacting protein|cbp HE|cytohesin binder and regulator|cytohesin binding protein HE|cytohesin-associated scaffolding protein|pleckstrin homology Sec7 and coiled-coil domains-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYTIP, check out the CYTIP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYTIP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cybr CYTIP Antibody (A09710)
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