Product Info Summary
SKU: | A04503 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-BORIS CTCFL Antibody
SKU/Catalog Number
A04503
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-BORIS CTCFL Antibody (Catalog # A04503). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store BORIS Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-BORIS CTCFL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04503)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04503 is reactive to CTCFL in Human
Applications
A04503 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
75747 MW
Background of BORIS
Anti-BORIS antibody is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as CCCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm. BORIS antibody can be used to investigate epigenetic regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
WB: 1:200 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of BORIS expression in human prostate extract (lane 1), and human spleen extract (lane 2). BORIS at 75KD was detected using rabbit anti-BORIS Antigen Affinity purified polyclonal antibody (Catalog # A04503). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For CTCFL (Source: Uniprot.org, NCBI)
Gene Name
CTCFL
Full Name
Transcriptional repressor CTCFL
Weight
75747 MW
Superfamily
CTCF zinc-finger protein family
Alternative Names
BORISHMGB1L1; BORIS-like protein; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC-binding factor (zinc finger protein)-like; CCCTC-binding factor; CT27MGC163358; CTCF paralog; CTCF-like protein; CTCF-T; dJ579F20.2; HMG-1L1; MGC169105; MGC169106; putative high mobility group protein 1-like 1; putative high mobility group protein B1-like 1; transcriptional repressor CTCFL; Zinc finger protein CTCF-T CTCFL BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2 CCCTC-binding factor like transcriptional repressor CTCFL|BORIS-like protein|brother of the regulator of imprinted sites|cancer/testis antigen 27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTCFL, check out the CTCFL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTCFL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BORIS CTCFL Antibody (A04503)
Hello CJ!
A04503 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A phase 1 study of weekly dosing of trastuzumab emtansine (T%u2010DM1) in patients with advanced human epidermal growth factor 2%u2013positive breast cancer
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-BORIS CTCFL Antibody
Question
Does anti-BORIS antibody A04503 work on horse WB with testis?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Our lab technicians have not validated anti-BORIS antibody A04503 on horse. You can run a BLAST between horse and the immunogen sequence of anti-BORIS antibody A04503 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-08
Question
Would anti-BORIS antibody A04503 work for WB with testis?
A. Miller
Verified customer
Asked: 2020-04-24
Answer
According to the expression profile of testis, CTCFL is highly expressed in testis. So, it is likely that anti-BORIS antibody A04503 will work for WB with testis.
Boster Scientific Support
Answered: 2020-04-24
Question
Do you have a BSA free version of anti-BORIS antibody A04503 available?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-BORIS antibody A04503 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-12
Question
I have a question about product A04503, anti-BORIS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04503 anti-BORIS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-BORIS antibody A04503. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-13
Question
See below the WB image, lot number and protocol we used for testis using anti-BORIS antibody A04503. Please let me know if you require anything else.
A. Mangal
Verified customer
Asked: 2019-03-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-14
Question
Is this A04503 anti-BORIS antibody reactive to the isotypes of CTCFL?
B. Roberts
Verified customer
Asked: 2018-10-05
Answer
The immunogen of A04503 anti-BORIS antibody is A synthetic peptide corresponding aa 9-26 of human BORIS protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-05
Question
I was wanting to use your anti-BORIS antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
As indicated on the product datasheet, A04503 anti-BORIS antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-26
Question
My question regards to test anti-BORIS antibody A04503 on human testis for research purposes, then I may be interested in using anti-BORIS antibody A04503 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Yang
Verified customer
Asked: 2017-10-23
Answer
The products we sell, including anti-BORIS antibody A04503, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-23
Question
We are currently using anti-BORIS antibody A04503 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
The anti-BORIS antibody (A04503) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-16
Question
Is a blocking peptide available for product anti-BORIS antibody (A04503)?
V. Carter
Verified customer
Asked: 2015-08-28
Answer
We do provide the blocking peptide for product anti-BORIS antibody (A04503). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-28
Question
Will A04503 anti-BORIS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Parker
Verified customer
Asked: 2015-02-02
Answer
As indicated on the product datasheet, A04503 anti-BORIS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-02
Question
I see that the anti-BORIS antibody A04503 works with WB, what is the protocol used to produce the result images on the product page?
J. Walker
Verified customer
Asked: 2014-09-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-22