Product Info Summary
SKU: | A11072 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-NADH-cytochrome b5 reductase 1 CYB5R1 Antibody
SKU/Catalog Number
A11072
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NADH-cytochrome b5 reductase 1 CYB5R1 Antibody (Catalog # A11072). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NADH-cytochrome b5 reductase 1 CYB5R1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11072)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CYB5R1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11072 is reactive to CYB5R1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
34095 MW
Background of CYB5R1
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction By similarity.
Zhu H., Proc. Natl. Acad. Sci. U.S.A. 96:14742-14747(1999).
Xu Z.G., Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Clark H.F., Genome Res. 13:2265-2270(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11072 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from RAW264.7 cells, using CYB5R1 antibody A11072.
Protein Target Info & Infographic
Gene/Protein Information For CYB5R1 (Source: Uniprot.org, NCBI)
Gene Name
CYB5R1
Full Name
NADH-cytochrome b5 reductase 1
Weight
34095 MW
Superfamily
flavoprotein pyridine nucleotide cytochrome reductase family
Alternative Names
NADH-cytochrome b5 reductase 1;b5R.1;1.6.2.2;Humb5R2;NAD (P)H:quinone oxidoreductase type 3 polypeptide A2;CYB5R1;NQO3A2;UNQ3049/PRO9865; CYB5R1 B5R.1, B5R1, B5R2, NQO3A2, humb5R2 cytochrome b5 reductase 1 NADH-cytochrome b5 reductase 1|NAD(P)H:quinone oxidoreductase type 3, polypeptide A2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYB5R1, check out the CYB5R1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYB5R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NADH-cytochrome b5 reductase 1 CYB5R1 Antibody (A11072)
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No publications found for A11072
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Customer Q&As
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4 Customer Q&As for Anti-NADH-cytochrome b5 reductase 1 CYB5R1 Antibody
Question
Is a blocking peptide available for product anti-CYB5R1 antibody (A11072)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-CYB5R1 antibody (A11072). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-CYB5R1 antibody A11072 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-13
Question
We are currently using anti-CYB5R1 antibody A11072 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
The anti-CYB5R1 antibody (A11072) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-14
Question
I have attached the WB image, lot number and protocol we used for platelet using anti-CYB5R1 antibody A11072. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-25